Black-Gold II Myelin Ready-to-Dilute Staining Kit with Toluidine Blue O Counter Stain

$597.00USD

Catalogue No.	TR-100-BG
Description	Black-Gold® II is a novel haloaurophosphate complex which localises myelin within the central nervous system. The Black Gold II Ready-to-Dilute (RTD™) Staining Kit allows you to localise myelin, both individual fibres and tracts, along with the option of co-localising cell bodies via the Toluidine Blue counter stain. Black Gold II labelled myelinated fibres appear nearly black while the Toluidine Blue O labelled cellular Nissl bodies are blue under bright field illumination. Black Gold II can demonstrate and characterise specific myelin changes associated with exposure to diverse neurotoxicants including kainic acid, domoic acid, 3-nitropropionic acid, Fluoro-Gold and isoniazid. Black Gold II can also be combined with other histochemical markers including Nissl stains, retrogradely transported fluorescent tracers and fluorescent markers of neuronal degeneration. The advantages associated with the Black-Gold II include high resolution, high contrast, short histochemical processing time, versatility and consistent reproducibility.
Applications	Black Gold II is a high resolution myelin stain for staining normal and pathological myelin. Its use is tailored to studies using formalin or paraformaldehyde fixed, non-paraffin embedded, non-solvent processed brain tissue.
Form	The reagents are all supplied in a liquid format and are ready-to-dilute.
Kit Components	Black-Gold II (Dilute 1:10 prior to use) - 10 mL Sodium Thiosulfate, fixative (Dilute 1:10 prior to use) - 10 mL Toluidine Blue O (Dilute 1:10 prior to use) - 10 mL Acetic Acid (Dilute 1:10 prior to use) - 10 mL
Reagent Kit protocol	TR-100-BG_February2012.pdf

Images (click to zoom)

Black-Gold II Myelin Ready-to-Dilute Staining Kit with Toluidine Blue O Counter Stain

Bright field illumination (60X mag) of the dentate gyrus of a normal mouse reveals individual Black-Gold II stained myelinated fibres and Toluadine Blue O stained polymorph cells (centre) and granule cells (top). Photo is courtesy of Dr. Larry Schmued.

Bright field illumination (60X mag) of 8 month old Ad-Tg mouse hippocampus. The myelin pathology can be observed in and around amyloid plaques. The cell bodies of adjacent granule and polymorph cells appear blue, while individual myeliated fibres appear nearly black. Photo is courtesy of Dr. Larry Schmued.

PDF Data Sheet	- Click to download the Data Sheet
MSDS	- Click to download the MSDS