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Chicken polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP)

$297.00USD


Catalogue No. C-1373-50
Description Glial fibrillary acidic protein (GFAP) is approx. 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Batch No. See product label
Unit size 50 l
Antigen Recombinant GFAP (expressed in E.coli) and native bovine GFAP
Isotype IgY
Other Names Astrocyte; Glial fibrillary acidic protein; GFAP
Accession P14136 GFAP_HUMAN; Q28115 GFAP_BOVIN;
Produced in Chicken
Molecular Weight On western blots of brain or spinal cord homogenate expect to see a band at 55 kDa and another at about 48 kDa, apparently a breakdown product of the primary band.
Applications Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:50,000 is recommended for WB. Human GFAP has a predicted length of 432 residues and a MW of 50 kDa. A dilution of 1:1000 using fluorescent secondary antibodies or 1:5000 using peroxidase or other enzyme-linked methods is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
ICC: 1:1000-1:20,000+, 4% PFA fixed cells in culture, 3hr-o/n incubations, optimization is a must in most cases because of the antibody high reactivity to GFAP
IHC: 4% Frozen tissues, permeabilized; IH(P): capable, HEIR treatment typically necessary; Chicken IgY can bind non-specifically to tissues, special chicken block solutions available elsewhere are recommended if used on tissues. Dilutions 1:5000 or greater; optimization on time, method required for best results.
Specificity The specificity of this antibody has been confirmed by WB.
Antibody Against Glial Fibrillary Acidic Protein
Cross-reactivity Human, Rat, Mouse, Feline. Predicted to react with other mammals.
Blast URL Click here
Form Lyophilised with 5% trehalose. The IgY preparation is at a concentration of ~25 mg/mL total protein.
Appearance White powder
Reconstitution Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage After reconstitution of lyophilised antibody, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
General References 1. Brenner M. et al (2001) Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet. 2001 Jan;27(1):117-20.
Images (click to zoom)
Chicken polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP) Mixed cultures of neurons and glia stained with Chicken polyclonal antibody to Glial Fibrillary Acidic Protein C-1373-50 (red) and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.

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