||Glial fibrillary acidic protein (GFAP) is approx. 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
||See product label
||Recombinant GFAP (expressed in E.coli) and native bovine GFAP
||Astrocyte; Glial fibrillary acidic protein; GFAP
||P14136 GFAP_HUMAN; Q28115 GFAP_BOVIN;
||On western blots of brain or spinal cord homogenate expect to see a band at 55 kDa and another at about 48 kDa, apparently a breakdown product of the primary band.
||Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:50,000 is recommended for WB. Human GFAP has a predicted length of 432 residues and a MW of 50 kDa. A dilution of 1:1000 using fluorescent secondary antibodies or 1:5000 using peroxidase or other enzyme-linked methods is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
ICC: 1:1000-1:20,000+, 4% PFA fixed cells in culture, 3hr-o/n incubations, optimization is a must in most cases because of the antibody high reactivity to GFAP
IHC: 4% Frozen tissues, permeabilized; IH(P): capable, HEIR treatment typically necessary; Chicken IgY can bind non-specifically to tissues, special chicken block solutions available elsewhere are recommended if used on tissues. Dilutions 1:5000 or greater; optimization on time, method required for best results.
||The specificity of this antibody has been confirmed by WB.
||Glial Fibrillary Acidic Protein
||Human, Rat, Mouse, Feline. Predicted to react with other mammals.
||Lyophilised with 5% trehalose. The IgY preparation is at a concentration of ~25 mg/mL total protein.
||Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
||After reconstitution of lyophilised antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
||12 months after purchase
||1. Brenner M. et al (2001) Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet. 2001 Jan;27(1):117-20.