| Catalogue No. |
C-1373-50 |
| Description |
Glial fibrillary acidic protein (GFAP) is approx. 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas. |
| Batch No. |
See product label |
| Unit size |
50 µl |
| Antigen |
Recombinant GFAP (expressed in E.coli) and native bovine GFAP |
| Isotype |
IgY |
| Other Names |
Astrocyte; Glial fibrillary acidic protein; GFAP |
| Accession |
P14136 GFAP_HUMAN; Q28115 GFAP_BOVIN; |
| Produced in |
Chicken |
| Applications |
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:50,000 is recommended for WB. Human GFAP has a predicted length of 432 residues and a MW of 50 kDa. A dilution of 1:1000 using fluorescent secondary antibodies or 1:5000 using peroxidase or other enzyme-linked methods is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
The specificity of this antibody has been confirmed by WB. |
| Antibody Against |
Glial Fibrillary Acidic Protein |
| Cross-reactivity |
Human, Rat, Mouse, Feline. Predicted to react with other mammals. |
| Blast URL |
Click here |
| Form |
Lyophilised with 5% trehalose |
| Appearance |
White powder |
| Reconstitution |
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution of lyophilised antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. |
| Expiry Date |
12 months after purchase |
| General References |
1. Brenner M. et al (2001) Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet. 2001 Jan;27(1):117-20.
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