biosensis fine bioscience tools - antibodies, elisa kits, proteins, toxins and peptides An Australia-US partnershipPhone: 1800 605 5127 in the US, or +61 8 8352 7711 for international enquiries to Australia.
HomeAbout BiosensisBiosensis distributorsResourcesHelp deskordering from BiosensisContact us
 


Technical Notes
 
View our tech notes
 
 
Follow us...
 
Google+ Follow us on Twitter

Find us on Facebook

Sign up for Biosensis newsletters
Visit our newsletter archive
 
 

Chicken polyclonal antibody to Microtubule Associated Protein 2 (MAP2)

$297.00USD


Catalogue No. C-1382-50
Description Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites.
Batch No. See product label
Unit size 50 l
Antigen Bovine MAP2 isolated from brain by the GTP microtubule cycling method.
Isotype IgY
Other Names Microtubule-associated protein 2; MAP-2; MAP2;
Accession P11137 MAP2_HUMAN;
Produced in Chicken
Applications Immunocytochemistry (IC) at suggested dilution of 1:2,500-5,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by IC.
Antibody Against Microtubule Associated Protein 2
Cross-reactivity Hu, Rat, Ms, Bov
Blast URL Click here
Form Lyophilised with 5% trehalose
Appearance White powder
Reconstitution Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage After reconstitution of lyophilised antibody, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Specific References Wolfes AC et al. (2016) "A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes."J Gen Physiol. 2016; [Epub ahead of print]. Application: IF Species: Rat

Dziennis S et al. (2007) "Role of signal transducer and activator of transcription-3 in estradiol-mediated neuroprotection". J Neurosci. 2007; 27(27):7268-74. Application: IHC Species: Rat
Images (click to zoom)
Chicken polyclonal antibody to Microtubule Associated Protein 2 (MAP2) View of mixed neuron/glial cultures stained with Chicken polyclonal antibody to Microtubule Associated Protein 2 C-1382-50 (red). The perikarya and dendrites of neurons are strongly and specifically stained with this antibody while the axons of the neurons and the processes of all other cell types in these cultures (astrocytes, oligodendrocytes, microglia, endothelia and fibroblasts) are all negative. Cell nuclei are visualized with DAPI DNA stain.
Chicken polyclonal antibody to Microtubule Associated Protein 2 (MAP2) View of a thin section of adult rat cerebellum stained with Chicken polyclonal antibody to Microtubule Associated Protein 2 C-1382-50 (green), Rabbit polyclonal to GFAP R-1374-50 (red) and DNA (blue). The image shows the molecular layer (outside of lobe) and granular layer; blue since its full of small neurons and the white matter in the middle.
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

MSDS

Click to download the MSDS - Click to download the MSDS

Offices in the USA and Australia
My Ice Bucket  
 
0 items
 
 
Currencies
 
  
 
 
Email This Page
 
 
Tell someone you know about this product.
 
 
We Accept PayPal
 
You can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_ID
Click to make a payment


Official PayPal Seal