||Dicer is the rate limiting enzyme in the formation of mature microRNAs.
||See product label.
||A synthetic peptide from mouse Dicer protein (1388-1405 aa) conjugated to KLH.
||Endoribonuclease Dicer; Double-strand-specific ribonuclease mDCR-1; Dicer1; Mdcr;
||Western Blotting (WB), Immunohistochemistry (IHC) and Immunoprecipitation (IP). The recommended concentration for IHC in formalin fixed and paraffin embedded tissues and formalin/acetone fixed tissues is 1:200-1:500. For WB, the recommended concentration is 1:1,000 to 1:3,000. Mouse Dicer Protein has a predicted length of 1916 residues and the MW of the monomer is 218 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||Toward understanding post-transcriptional gene expression From purple petunias to the brain and memory; slicing and dicing our way toward understanding post-transcriptional gene expression ...read more...
||Specificity confirmed by IHC and WB in mouse brain.
||Lyophilised from PBS, 0.02% sodium azide
||Reconstitute in 100 µl of sterile distilled water. Centrifuge to remove any insoluble material.
||At least 12 months after purchase at 2 - 4°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
||12 months after purchase
||1. Lugli G. et al (2008) Expression of microRNAs and their precursors in synaptic fractions of adult mouse forebrain.
J Neurochem. Jul;106(2):650-61.
2. Lugli G. et al (2005) Dicer and eIF2c are enriched at postsynaptic densities in adult mouse brain and are modified by neuronal activity in a calpain-dependent manner.
J Neurochem. Aug;94(4):896-905.