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Chicken polyclonal antibody to mouse Dicer protein (1388-1405): Affinity purified

$297.00USD


Catalogue No. C-1557-100
Description Dicer is the rate limiting enzyme in the formation of mature microRNAs.
Batch No. See product label.
Unit size 100 l
Antigen A synthetic peptide from mouse Dicer protein (1388-1405 aa) conjugated to KLH.
Sequence C-NQDKSNSEKWEKDEMTKD
Other Names Endoribonuclease Dicer; Double-strand-specific ribonuclease mDCR-1; Dicer1; Mdcr;
Accession Q8R418 DICER_MOUSE;
Produced in Chicken
Applications Western Blotting (WB), Immunohistochemistry (IHC) and Immunoprecipitation (IP). The recommended concentration for IHC in formalin fixed and paraffin embedded tissues and formalin/acetone fixed tissues is 1:200-1:500. For WB, the recommended concentration is 1:1,000 to 1:3,000. Mouse Dicer Protein has a predicted length of 1916 residues and the MW of the monomer is 218 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Featured Articles Toward understanding post-transcriptional gene expression From purple petunias to the brain and memory; slicing and dicing our way toward understanding post-transcriptional gene expression ...read more...
Specificity Specificity confirmed by IHC and WB in mouse brain.
Cross-reactivity Mouse; Rat;
Blast URL Click here
Form Lyophilised from PBS, 0.02% sodium azide
Reconstitution Reconstitute in 100 l of sterile distilled water. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 4C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability and at 4C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Specific References 1. Lugli G. et al (2008) Expression of microRNAs and their precursors in synaptic fractions of adult mouse forebrain. J Neurochem. Jul;106(2):650-61.
2. Lugli G. et al (2005) Dicer and eIF2c are enriched at postsynaptic densities in adult mouse brain and are modified by neuronal activity in a calpain-dependent manner. J Neurochem. Aug;94(4):896-905.
Images (click to zoom)
Chicken polyclonal antibody to mouse Dicer protein (1388-1405): Affinity purified Western blotting with Chicken polyclonal antibody to mouse Dicer protein (C-1557-100) in mouse brain extracts. Figure A: Mouse brain cytosolic extract. Detection at 0.03µg/ml. Protein loaded 20µg, 10µg and 5µg. Figure B: Mouse brain subcellular fractionation. Detection at 0.03µg/ml. Protein loaded 10µg.

PDF Data Sheet

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MSDS

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