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Chicken polyclonal antibody to Peripherin

$297.00USD


Catalogue No. C-1399-50
Description Peripherin is a class-III neuronal intermediate filament protein found in certain classes of neuron, most of which are located in the peripheral nervous system.
Batch No. See product label
Unit size 50 µl
Antigen Recombinant full length rat Peripherin protein expressed in and purified from E.coli
Isotype IgY
Other Names Peripherin; Prph; Prph1;
Accession P21807 PERI_RAT;
Produced in Chicken
Applications Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB. This antibody detects ~57 kDa Peripherin protein. A suitable control tissue is rat spinal cord or peripheral nerve homogenate.
Antibody Against Peripherin
Cross-reactivity Hu, Rat, Ms, Fel, and other mammals
Blast URL Click here
Form Lyophilised with 5% trehalose
Appearance White powder
Reconstitution Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage After reconstitution of lyophilised antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Specific References 1. Sekerková G. et al (2008) Espin actin-cytoskeletal proteins are in rat type I spiral ganglion neurons and include splice-isoforms with a functional nuclear localization signal. J Comp Neurol. 2008 Aug 20;509(6):661-76.
Images (click to zoom)
Chicken polyclonal antibody to Peripherin Background image: Substantia Nigra neurons from QBM CellScience thawed and cultured for 5 days and stained with Biosensis rabbit antibody to Tyrosine Hydroxylase (red, Catalog Number R-148-50) and monoclonal antibody Tuj (green). Photo courtesy of QBM Cell Science. Left and middle image: Dorsal Root Ganglia neurons from QBM CellScience thawed and cultured for 7 days and stained with Biosensis chicken antibody to Peripherin (red, Catalog Number C-1399-50) and monoclonal antibody Tuj (green). Photo courtesy of QBM Cell Science. Right image: Substantia Nigra neurons from QBM CellScience thawed and cultured for 14 days and stained with Biosensis rabbit antibody to NeuN (red, Catalog Number R-3770-100) and monoclonal antibody Tuj (green). Photo courtesy of QBM Cell Science.
Chicken polyclonal antibody to Peripherin Image shows rat mixed neuron/glial cultures stained with Chicken polyclonal antibody to Peripherin C-1399-50 (green channel) and Rabbit polyclonal antibody to Internexin alpha R-1379-50 (green channel). These cultures contain mostly neurons which are rich in internexin-alpha, and a subgroup which have a large amount of peripherin also, such as the prominent cell in the middle of the micrograph. Since this cell expresses large amounts of peripherin and internexin-alpha, the green and red signals superimpose to produce a golden cell. Blue is a DNA stain.
PDF Data Sheet

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MSDS

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