| Catalogue No. |
C-1406-50 |
| Description |
This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT). |
| Batch No. |
See product label |
| Unit size |
50 µl |
| Antigen |
Recombinant full length human Ubiquitin C Terminal Hydrolase 1 (UCHL1) purified from E. coli. |
| Isotype |
IgY |
| Other Names |
Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1; |
| Accession |
P09936 UCHL1_HUMAN; |
| Produced in |
Chicken |
| Applications |
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
The specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord or peripheral nerve homogenate. |
| Antibody Against |
Ubiquitin C Terminal Hydrolase 1 |
| Cross-reactivity |
Hu, Rat, Ms, Bov, Por. Predicted to react with other mammalian tissues due to sequence homology. |
| Blast URL |
Click here |
| Form |
Lyophilised with 5% trehalose |
| Appearance |
White powder |
| Reconstitution |
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution of lyophilised antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. |
| Expiry Date |
12 months after purchase |
