Chicken polyclonal antibody to Vimentin

$287.00USD

Catalogue No.	C-1409-50
Description	Vimentins are class-III intermediate filaments specific to mesenchymal tissue. Vimentin is an important cytoskeletal component responsible for maintaining cell integrity and has a probable role in the intracellular transport of proteins such as lipoproteins between the nucleus and plasma membrane. Immunohistochemical staining for Vimentin is characteristic of sarcomas.
Batch No.	See product label
Unit size	50 µl
Antigen	Recombinant human Vimentin purified from E.coli
Isotype	IgY
Other Names	VIM;
Accession	P08670 VIME_HUMAN;
Produced in	Chicken
Applications	Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000-5,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity	The specificity of this antibody has been confirmed by WB. This antibody detects ~50 kDa Vimentin enzyme.
Antibody Against	Vimentin
Cross-reactivity	Hu, Rat, Ms. It is predicted to react with other mammals due to sequence homology.
Blast URL	Click here
Form	Lyophilised with 5% trehalose
Appearance	White powder
Reconstitution	Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage	After reconstitution of lyophilised antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date	12 months after purchase

Images (click to zoom)

View of mixed neuron/glial cultures stained with Chicken polyclonal antibody to Vimentin C-1409-50 (green) and Rabbit polyclonal antibody to Glial Fibrillary Acidic Protein R-1374-50 (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate which appear green. Astrocytes may express primarily Glial Fibrillary Acidic Protein (GFAP), or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments.

A view of neonatal rat brain cultures stained with Chicken polyclonal antibody to Vimentin C-1409-50 (red) and with Rabbit polyclonal antibody to GFAP R-1374-50 (green). These two proteins are found only in non-neuronal cells so you can't see any neurons, except for their nuclei in blue. Maturish astrocytes have only GFAP so appear green or have a mix of both proteins, so appear yellow. Some cells only have the vimentin (immature astrocytes, microglia and fibroblasts) and so appear red.

PDF Data Sheet	- Click to download the Data Sheet
MSDS	- Click to download the MSDS