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Fluoro-Jade C (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating Neurons

$397.00USD


Catalogue No. TR-100-FJ
Description The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. Fluoro-Jade® C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.
Applications The Fluoro-Jade C 'Ready to Dilute' (RTD™) Staining Kit provides an easy to use assortment of Fluoro-Jade C, DAPI, sodium hydroxide and potassium permanganate in liquid form. Following our detailed protocol, Fluoro-Jade C labelled degenerating neurons are visualised with blue light excitation while DAPI counter stained cell nuclei are visualised with ultra-violet illumination. The Fluoro-Jade C Staining Kit can be used on all kinds of preserved tissues, including fresh-frozen, paraformaldehyde or formalin fixed, and formalin fixed, paraffin-embedded tissues.
Comments MATERIALS PROVIDED

Sodium Hydroxide, Solution A (Dilute 1:10 prior to use) - 40 mL
Potassium Permanganate, Solution B (Dilute 1:10 prior to use) - 40 mL
Fluoro-Jade C, Solution C (Dilute 1:10 prior to use) - 40 mL
DAPI, Solution D (Add to diluted Fluoro-Jade C) - 40 mL


EQUIPMENT AND REAGENTS NEEDED

Gelatin coated microscope slides
Staining dishes/Coplin jars
Cover slips
DPX mounting media
Slide warmer
Convection oven
Distilled water
Ethanol
Xylene

NUMBER OF SLIDES PROCESSED:

The actual number of slides processed by this kit will depend largely upon the vessel that is used to incubate the slides. If using a standard Coplin Jar, its capacity is 50 mL and typically holds 5 slides per jar. If using such a device, then 80-100 slides stained per 50 ml of working solution (or, 5 ml of stock solution) could be processed in one day. Note the diluted dye is NOT stable and will not store overnight. It is best to use freshly diluted dye each time an experimental batch is started.

Final working concentrations of FJC:0.0001%
Final working concentration of KMnO4: 0.06%
Specificity Degenerating neurons, and neuronal degeneration. There is no specific staining in normal healthy brain.
Cross-reactivity Note: Some researchers under some conditions report blood vessel staining with Fluoro Jade. This may be because Fluoro Jade is an analogue of eosin (which stains blood cells). In general, good perfusion and preparation of the tissue should help prevent blood vessel staining but it may not be possible to eliminate it entirely. In our experience it is generally possible to distinguish neuronal from blood vessels staining by eye.
Form The reagents in the Fluoro Jade kit are all supplied in a liquid format and are ready-to-dilute.
Appearance FJC visualization is accomplished using blue light or a 488nm Laser. Excitation Peak: 495 nm Emission Peak: 521 nm Filter system for visualizing: Fluorescein/FITC
Reconstitution Dilute solutions as directed in the protocol instructions. Sometimes small precipitates may be present in the stock or diluted solutions. Complete mixing of the diluted solutions usually dissolves the precipitates. The precipitates, if not removed, do not usually cause any difficulties if the washing steps are followed as instructed. Optional: For entirely clean solutions Biosensis recommends filtering the diluted solution though ethanol and NaOH compatible syringe or vacuum filter devices prior to contact with tissue slides.
Storage The unopened kit can be stored for up to 6 months at 2-8ºC after the date of receipt. The kit and components should be stored protected from light. Diluted FJC dye solutions are not stable and should be used within 4 hours of making. The other diluted solutions can be reused and stored for up to 48 hours if refrigerated and protected from light. Best results require freshly diluted solutions. We recommend using aseptic techniques when handling the reagents to avoid bacterial growth and contamination.

The FJC Ready to dilute kit is shipped ambient and stable at room temperature during transport. Refrigerate upon arrival, do not freeze.
Expiry Date Unopened kit 6 months at 2-8ºC protected from light. See Storage instructions for working solutions recommendations.
Specific References Kononenko NL, Claßen GA, Kuijpers M, Puchkov D, Maritzen T, Tempes A, Malik AR, Skalecka A, Bera S, Jaworski J, Haucke V (2017) "Retrograde transport of TrkB-containing autophagosomes via the adaptor AP-2 mediates neuronal complexity and prevents neurodegeneration." Nat Commun. 8:14819 Application: IF. Species: Mouse

Lian YD, Chen ZX, Zhu KR, Sun SY, Zhu LP (2017) "Effect of equipotent doses of bupivacaine and ropivacaine in high‐fat diet fed neonatal rodent model." Braz J Anesthesiol. 67(2):131-8 Application: IF. Species: Rat

Mamo JCL, Lam V, Giles C, Coulson SH, Fimognari N, Mooranian A, Al-Salami H, Takechi R (2017) "Antihypertensive agents do not prevent blood–brain barrier dysfunction and cognitive deficits in dietary-induced obese mice." Int J Obes (Lond). [Epub ahead of print] Application: IF. Species: Mouse

Iwasa K, Yamamoto S, Yagishita S, Maruyama K, Yoshikawa K (2017) "Excitotoxicity-induced prostaglandin D2 production induces sustained microglial activation and delayed neuronal death." J Lipid Res. 58(4):649-55 Application: IF. Species: Rat

Xie Z, Huang L, Enkhjargal B, Reis C, Wan W, Tang J, Cheng Y, Zhang JH (2017) "Intranasal administration of recombinant Netrin-1 attenuates neuronal apoptosis by activating DCC/APPL-1/AKT signaling pathway after subarachnoid hemorrhage in rats." Neuropharmacology. http://dx.doi.org/10.1016/j.neuropharm.2017.03.025 [In press] Application: IF. Species: Rat

Katayama Y, Inaba T, Nito C, Suda S, Ueda M (2016) "Neuroprotective effects of clarithromycin against neuronal damage in cerebral ischemia and in cultured neuronal cells after oxygen-glucose deprivation." Life Sci. PMID:27825902 [Epub ahead of print] Application: IF. Species: Rat

Yang LY, Greig NH, Huang YN, Hsieh TH, Tweedie D, Yu QS, Hoffer BJ, Luo Y, Kao YC, Wang JY (2016) "Post-traumatic administration of the p53 inactivator pifithrin-α oxygen analogue reduces hippocampal neuronal loss and improves cognitive deficits after experimental traumatic brain injury." Neurobiol Dis. PMID:27553877 [Epub ahead of print] Application: IF. Species: Rat

Izumida H, Takagi H, Fujisawa H, Iwata N, Nakashima K, Takeuchi S, Iwama S, Namba T, Komatu Y, Kaibuchi K, Oiso Y, Arima H, Sugimur Y (2016) "NMDA receptor antagonist prevents cell death in the hippocampal dentate gyrus induced by hyponatremia accompanying adrenal insufficiency in rats." Exp Neurol. PMID:27527984 [Epub ahead of print] Application: IF. Species: Rat

Dixon BJ, Chen D, Zhang Y, Flores J, Malaguit J, Nowrangi D, Zhang JH, Tang J. (2016) "Intranasal Administration of Interferon Beta Attenuates Neuronal Apoptosis via the JAK1/STAT3/BCL-2 Pathway in a Rat Model of Neonatal Hypoxic-Ischemic Encephalopathy." ASN Neuro. PMID:27683877 8(5) Application: IF. Species: Rat

Wang JY, Huang YN, Chiu CC, Tweedie D, Luo W, Pick CG, Chou SY, Luo Y, Hoffer BJ, Greig NH, Wang JY (2016) "Pomalidomide mitigates neuronal loss, neuroinflammation, and behavioral impairments induced by traumatic brain injury in rat." J Neuroinflammation. PMID:27353053 13(1):168 Application: IF. Species: Rat

Wang G, Manaenko A, Shao A, Ou Y, Yang P, Budbazar E, Nowrangi D, Zhang JH, Tang J (2016) "Low-density lipoprotein receptor-related protein-1 facilitates heme scavenging after intracerebral hemorrhage in mice." J Cereb Blood Flow Metab. PMID:27317656 [Epub ahead of pring] Application: IF. Species: Mouse

Safdaria BK, Siab TC, Wattchow DA, Smid SD (2016) "Effects of pro-inflammatory cytokines, lipopolysaccharide and COX-2 mediators on human colonic neuromuscular function and epithelial permeability." Cytokine. PMID:27177092 83:231-8 Application: IF. Species: Human

Lian YD, Chen ZX, Zhu KR, Sun SY, Zhu LP (2016) "Effect of equipotent doses of bupivacaine and ropivacaine in high-fat diet fed neonatal rodent model." Brazilian Journal of Anesthesiology [In Press] Application: IF. Species: Rat

Launay PS, Reboussin E, Liang H, Kessal K, Godefroy D, Rostene W, Sahel JA, Baudouin C, Parsadaniantz SM, Le Goazigo AR (2016) "Ocular inflammation induces trigeminal pain, peripheral and central neuroinflammatory mechanisms." Neurobiology of Disease. PMID:26747211 88:16-28 Application: IH. Species: Mouse

Khan D, Dupper A, Deshpande T, Graan PN, Steinhäuser C, Bedner P. (2016) "Experimental febrile seizures impair interastrocytic gap junction coupling in juvenile mice." J Neurosci Res. PMID:26931373 [Epub ahead of print] Application: IF. Species: Mouse

Bye N, Christie KJ, Turbic A, Basrai HS, Turnley AM (2016) "Rho kinase inhibition following traumatic brain injury in mice promotes functional improvement and acute neuron survival but has little effect on neurogenesis, glial responses or neuroinflammation." Exp Neurol. PMID:26896832 [Epub ahead of print] Application: IH. Species: Mouse

Bedner P, Dupper A, Hüttmann K, Müller J, Herde MK, Dublin P, Deshpande T, Schramm J, Häussler U, Haas CA, Henneberger C, Theis M, Steinhäuser C (2015) "Astrocyte uncoupling as a cause of human temporal lobe epilepsy." Brain. PMID:25765328 [Epub ahead of print] Application: IH 4%PFA, vibratome 40um sections. Species: Mouse

Ramesh G, Didier PJ, England JD, Santana-Gould L, Doyle-Meyers LA, Martin DS, Jacobs MB, Philipp MT (2015) "Inflammation in the Pathogenesis of Lyme Neuroborreliosis." Am J Pathol. PMID:25892509 [Epub ahead of print] Application: IH with Z-fixative, 5 micron sections. Species: Rhesus Monkey

Suda S, Ueda M, Nito C, Nishiyama Y, Okubo S, Abe A, Aoki J, Suzuki K, Sakamoto Y, Kimura K (2015) "Valproic acid ameliorates ischemic brain injury in hyperglycemic rats with permanent middle cerebral occlusion." Brain Res. PMID:25721785 1606:1-8 Application: IH. Species: Rat

Mayeux JP, Teng SX, Katz PS, Gilpin NW, Molina PE (2015) "Traumatic brain injury induces neuroinflammation and neuronal degeneration that is associated with escalated alcohol self-administration in rats." Behav Brain Res. PMID:25446758 279:22-30 Application: IH. Species: Rat

Yang LY, Chu YH, Tweedie D, Yu QS, Pick CG, Hoffer BJ, Greig NH, Wang JY (2015) "Post-trauma administration of the pifithrin-α oxygen analog improves histological and functional outcomes after experimental traumatic brain injury." Exp Neurol. PMID:25819102 269:56-66 Application: IH. Species: Rat

Ren YY, Zhang HQ, Duan SH, Wang SD (2015) "Influence of Dexmedetomidine on Toxicity of Intrathecal Ketamine on Neonatal Rat Spinal Function." Tropical Journal of Pharmaceutical Research. 14(3):469-77 Application: IH. Species: Rat

Iseli CE, Merwin WH 3rd, Klatt-Cromwell C, Hutson KA, Ewend MG, Adunka OF, Fitzpatrick DC, Buchman CA (2014) "Effect of Cochlear Nerve Electrocautery on the Adult Cochlear Nucleus." Otol Neurotol. PMID: 25280052 [Epub ahead of print] Application: IH(P)

Saito T, Nito C, Ueda M, Inaba T, Kamiya F, Muraga K, Katsura K, Katayama Y (2014) "Continuous oral administration of atorvastatin ameliorates brain damage after transient focal ischemia in rats." Life Sci. PMID: 24333133 94(2):106-14 Application: IH. Species: Rat

Hayashida K, Sano M, Kamimura N, Yokota T, Suzuki M, Ohta S, Fukuda K, Hori S (2014) "Hydrogen inhalation during normoxic resuscitation improves neurological outcome in a rat model of cardiac arrest independently of targeted temperature management." Circulation. PMID: 25366995 130(24):2173-80 Application: IH. Species: Rat
Reagent Kit protocol Click to download the protocolTR-100-FJ_January2017_protocol.pdf
MSDS Click to download the Material Safety Data SheetMSDS_TR-100-FJ_as_at_May2017.pdf
Images (click to zoom)
Fluoro-Jade C  (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating Neurons Double exposure using combined blue and ultraviolet epi-fluorescent illumination of the superficial layers of the cingulated rat cortex exposed to kainic acid. Layer I contains conspicuous Fluoro-Jade C positive degenerating axon terminals. Layer II contains densely packed DAPI-positive viable granule cells. Layer III contains a mixture of Fluoro-Jade C positive denegerating pyramidal cells and DAPI-positive viable pyramidal cells. Photo is courtesy of Dr. Larry Schmued.
Fluoro-Jade C  (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating Neurons Triple exposure combining ultraviolet, blue and green light epi-fluorescent illumination (10X) of rat hippocampus exposed to kainic acid. The section was triple labeled with Fluoro-Jade C and DAPI staining combined with GFAP immunohistochemistry. The section reveals extensive green Fluoro-Jade C positive neuronal degeneration throughout the entire CA-1 region of the hippocampus. The underlying blue viable positive granule cells of the dentate gyrus are only DAPI positive. Both regions exhibit red GFAP positive hypertrophied astrocytes. Photo is courtesy of Dr. Larry Schmued.
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

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