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Fluoro-Jade C Ready-to-Dilute Staining Kit for identifying Degenerating Neurons

$397.00USD


Catalogue No. TR-100-FJ
Description The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. Fluoro-Jade® C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.
Applications The Fluoro-Jade C 'Ready to Dilute' (RTD™) Staining Kit provides an easy to use assortment of Fluoro-Jade C, DAPI, sodium hydroxide and potassium permanganate in liquid form. Following our detailed protocol, Fluoro-Jade C labelled degenerating neurons are visualised with blue light excitation while DAPI counter stained cell nuclei are visualised with ultra-violet illumination. The Fluoro-Jade C Staining Kit can be used on all kinds of preserved tissues, including fresh-frozen, paraformaldehyde or formalin fixed, and formalin fixed, paraffin-embedded tissues.
Form The reagents in the Fluoro Jade kit are all supplied in a liquid format and are ready-to-dilute.
Reconstitution Dilute solutions as directed in the protocol instructions. Sometimes small precipitates may be present in the stock or diluted solutions. Complete mixing of the diluted solutions usually dissolves the precipitates. The precipitates, if not removed, do not usually cause any difficulties if the washing steps are followed as instructed. Optional: For entirely clean solutions Biosensis recommends filtering the diluted solution though ethanol and NaOH compatible syringe or vacuum filter devices prior to contact with tissue slides.
Storage The kit can be transported at room temperature. Once received, the kit can be stored for up to 12 months at 4°C protected from light. Diluted solutions can be stored up to one month at 4°C protected from light.
Kit Components Sodium Hydroxide (Dilute 1:10 prior to use) - 40 mL Potassium Permanganate (Dilute 1:10 prior to use) - 40 mL Fluoro-Jade C (Dilute 1:10 prior to use) - 40 mL DAPI (Add to diluted Fluoro-Jade C) - 40 mL
Reagent Kit protocol Click to download the protocolTR-100-FJ 08-02-2013.pdf
MSDS Click to download the Material Safety Data SheetMSDS_TR-100-FJ_as_at_Apr2014.pdf
Images (click to zoom)
Fluoro-Jade C Ready-to-Dilute Staining Kit for identifying Degenerating Neurons Double exposure using combined blue and ultraviolet epi-fluorescent illumination of the superficial layers of the cingulated rat cortex exposed to kainic acid. Layer I contains conspicuous Fluoro-Jade C positive degenerating axon terminals. Layer II contains densely packed DAPI-positive viable granule cells. Layer III contains a mixture of Fluoro-Jade C positive denegerating pyramidal cells and DAPI-positive viable pyramidal cells. Photo is courtesy of Dr. Larry Schmued.
Fluoro-Jade C Ready-to-Dilute Staining Kit for identifying Degenerating Neurons Triple exposure combining ultraviolet, blue and green light epi-fluorescent illumination (10X) of rat hippocampus exposed to kainic acid. The section was triple labeled with Fluoro-Jade C and DAPI staining combined with GFAP immunohistochemistry. The section reveals extensive green Fluoro-Jade C positive neuronal degeneration throughout the entire CA-1 region of the hippocampus. The underlying blue viable positive granule cells of the dentate gyrus are only DAPI positive. Both regions exhibit red GFAP positive hypertrophied astrocytes. Photo is courtesy of Dr. Larry Schmued.
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

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