| Catalogue No.: |
GP-030-50 |
| Batch No.: |
See product label |
| Unit size: |
50 µl |
| Antigen: |
A synthetic peptide corresponding to the antigenic region within the human alpha MSH residue. This peptide was conjugated to carrier protein to enhance the immunological response. |
| Other Names: |
Proopiomelanocortin; POMC; alpha-melanocyte stimulating hormone; melanotropin alpha |
| Accession: |
COLI_HUMAN |
| Description: |
Proopiomelanocortin (POMC) is the precursor of the melanocortin peptides alpha, beta and gamma. POMC neurons in the arcuate nucleus of the hypothalamus function as key central regulators of food intake and homeostatic control of energy balance in both rodents and non-human primates. MSH increases the pigmentation of skin by increasing the melanin production in melanocytes. MSH is produced in the pituitary gland and belongs to the POMC family. |
| Produced in: |
Guinea pig |
| Purity: |
Neat serum |
| Applications: |
IHC. A concentration of 1 in 2000 is recommended for IHC. IHC performed in sheep brain (hypothalamus) demonstrates intense staining of cells. No staining is evident when the primary antibody is pre-absorbed with 0.5mg/ml of alpha MSH. Cells are stained when the primary antibody is pre-absorbed with 0.5mg/ml of beta MSH or 0.5mg/ml of gamma MSH. The optimal working dilution should be determined by the end user. |
| Specificity: |
Specificity was demonstrated by immunohistochemistry. |
| Cross-react: |
This antibody is known to react with sheep. Other species have not yet been tested. |
| Blast it: |
If you would like to see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it. |
| Form: |
Lyophilised |
| Reconstitution: |
Reconstitute in 50 µl sterile water. Centrifuge to remove any insoluble material. |
| Storage: |
It is recommended that a thawed sample is stored at 4ºC for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20ºC or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this. |
| Expiry Date: |
12 months upon reconstitution. |
| References: |
Grayson BE, Allen SE, Billes SK, Williams SM, Smith MS, Grove KL (2006). Prenatal development of hypothalamic neuropeptide systems in the nonhuman primate.
J Neuroscience. 143(4) pp. 975-86. |
