| Catalogue No. |
GP-036-50 |
| Description |
Luteinizing hormone promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. It exists as a heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin, and gonatropin. |
| Batch No. |
See product label |
| Unit size |
50 µl |
| Antigen |
A synthetic peptide corresponding to the antigenic region within the ovine LH residue. |
| Other Names |
Lutropin subunit beta [Precursor]; Luteinizing hormone subunit beta; LSH-beta; LSH-B; LH-B; Lutropin beta chain; Interstitial cell-stimulating hormone |
| Accession |
LSHB_SHEEP |
| Produced in |
Guinea pig |
| Purity |
Neat serum. |
| Applications |
IHC. A concentration of 1 in 3000 is recommended for IHC. IHC performed in sheep pituitary demonstrates intense staining of cells. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/ml of LH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
Specificity was demonstrated by immunohistochemistry. |
| Cross-reactivity |
This antibody is known to react with sheep. Other species have not yet been tested |
| Blast it |
If you would like to see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it. |
| Form |
Lyophilised |
| Reconstitution |
Reconstitute in 50 µl sterile water. Centrifuge to remove any insoluble material. |
| Storage |
It is recommended that a thawed sample is stored at 4ºC for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20ºC or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this. |
| Expiry Date |
12 months upon reconstitution. |
| References |
Clarke IJ, Tobin VA, Pompolo S, Pereira A (2005). Effects of changing Gonadotropin-releasing hormone pulse frequency and estrogen treatment on levels of estradiol receptor –alpha and induction of Fos and phosphorylated cyclic adenosine monophosphate response element binding protein in pituitary gonadotropes: studies in hypothalamo-pituitary disconnected ewes. Endocrinology 146 (3) pp. 1128-1137. |
