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Guinea pig antibody to oxytocin: whole serum

$245.00USD

Catalogue No.: GP-037-50
Batch No.: See product label
Unit size: 50 µl
Antigen: A synthetic peptide corresponding to the antigenic region within the oxytocin hormone residue. This peptide was conjugated to carrier protein to enhance the immunological response.
Other Names: OT
Accession: NEU1_HUMAN
NEU1_SHEEP
NEU1_BOVIN
NEU1_MOUSE
NEU1_RAT
Description: Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. It belongs to the vasopressin/oxytocin family. Oxytocin is secreted.
Produced in: Guinea pig
Purity: Neat serum.
Applications: IHC. A concentration of 1 in 2000 is recommended for IHC. IHC performed in sheep brain (hypothalamus) demonstrates intense staining of cells and terminals. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/ml of oxytocin. The optimal working dilution should be determined by the end user.
Specificity: Specificity was demonstrated by immunohistochemistry.
Cross-react: This antibody is known to react with sheep. Other species have not yet been tested.
Blast it: If you would like to see the shared identity between different species or other proteins follow the link in the Accession field, select the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it.
Form: Lyophilised
Reconstitution: Reconstitute in 50 µl sterile water. Centrifuge to remove any insoluble material.
Storage: It is recommended that a thawed sample is stored at 4ºC for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20ºC or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Expiry Date: 12 months upon reconstitution.
References: Thompson MR, Callaghan PD, Hunt GE, Cornish JL, Mcgregor IS. (2007). A role for oxytocin and 5-HT1A receptors in the prosocial effects of 3,4 Methylendioxymethamphetamine (�ecstasy�). Neuroscience. 146(2) pp. 509-14.
Related items: GP-029-50, GP-030-50, GP-031-50, GP-032-50, GP-036-50
Images
Guinea pig antibody to oxytocin: whole serum The cryostat section of the sheep hypothalamus was incubated in guinea pig polyclonal antibodies to oxytocin at the dilution of 1:1000 overnight followed by incubation with biotinylated secondary antibodies. Cell bodies and nerve terminals (from cells that produce this hormone) in the sheep brain are intensely stained. This figure shows staining of cells when no pre-absorption is performed.

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