| Catalogue No. |
GP-032-50 |
| Description |
Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) and upon binding to the receptor it induces the release of growth hormone from the pituitary. This ligand has an appetite-stimulating effect and is involved in growth regulation. |
| Batch No. |
See product label |
| Unit size |
50 µl |
| Antigen |
A synthetic peptide (GSSFLSPEHQRVQQRKESKKPPAKLQPR) corresponding to the amino acids 24-51 from human ghrelin. This peptide was conjugated to carrier protein to enhance the immunological response. |
| Other Names |
Appetite-regulating hormone; Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; M46 protein |
| Accession |
GHRL_HUMAN |
| Produced in |
Guinea pig |
| Purity |
Neat serum. |
| Applications |
IHC. A concentration of 1 in 3000 is recommended for IHC. IHC performed in sheep abomasum demonstrates intense staining of cells and terminals. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/ml of human ghrelin. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
Specificity was demonstrated by immunohistochemistry. |
| Cross-reactivity |
This antibody is known to react with sheep and human. Other species have not yet been tested. |
| Blast it |
If you would like to see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it. |
| Form |
Lyophilised |
| Reconstitution |
Reconstitute in 50 µl sterile water. Centrifuge to remove any insoluble material. |
| Storage |
It is recommended that a thawed sample is stored at 4°C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20°C or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this. |
| Expiry Date |
12 months upon reconstitution. |
| References |
Kojima M, Hosoda H, Matsuo H, Kangawa K (2001). Ghrelin; discovery of the natural endogenous ligand for the growth hormone secretagogue receptor.
Trends Endocrinol Metab 12 pp 118-122. |
