| Catalogue No. |
PE-1239-5 |
| Description |
Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. |
| Batch No. |
See product label |
| Unit size |
5 µg |
| Other Names |
Beta-nerve growth factor; beta-NGF; NGFB; |
| Accession |
P01138 NGF_HUMAN; |
| Produced in |
A DNA sequence encoding the human beta NGF protein sequence (containing the signal peptide, pro-peptide and the mature beta NGF sequence) was expressed in modified human 293 cells.
Theoretical peptide sequence:
YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEAR
PVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT |
| MW |
The NGF protein migrates at approximately 12-16 kDa in SDS-PAGE. It has a predicted molecular mass of 13.5 kDa. The protein separates into a number of isoforms with a pI between 9 and 10 in 2D PAGE due to post-translational modifications. The unmodified protein has a predicted pI of 9. |
| Purity |
>95%, as determined by SDS-PAGE and visualized by silver stain |
| Biol. activity |
The ED50 of beta NGF is typically 0.2-1.0 ng/ml as measured in a cell proliferation assay using the human growth factor dependent TF-1 cell line. |
| Form |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term
storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C.
Repeated freeze thawing is not recommended. |
