| Catalogue No. |
PE-1240-25 |
| Description |
Epidermal Growth Factor (EGF) Receptor binds to EGF and other members of the EGF family such as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. At least 4 isoforms are produced by alternative splicing (reference SWISSPROT). The EGF receptor is a single-pass type I membrane protein containing an extracellular receptor domain, a transmembrane region and an intracellular domain with tyrosine kinase function. |
| Batch No. |
See product label |
| Unit size |
25 µg |
| Other Names |
Receptor tyrosine-protein kinase erbB-1; EGFR; Urogastrone; Pro-epidermal growth factor; EGF R; c-erbB-1; ERBB1; |
| Accession |
P00533 EGFR_HUMAN; |
| Produced in |
A DNA sequence encoding the signal peptide and extracellular domain of human Epidermal Growth Factor Receptor (amino acids 1-525) was fused to the Fc region of human IgG1 (amino acids 90-330). The chimeric protein was expressed in modified human 293 cells. |
| MW |
Under reducing conditions, the EGF receptor-Fc chimera migrates as a broad band
between 85 and 110 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. The unmodified EGF receptor-Fc chimera has a predicted
monomeric molecular mass of 83 kDa.
The chimera separates into a number of glycoforms with a pI between 6.0 and 8.5 on 2D PAGE. The unmodified chimera has a predicted pI of 7.8. |
| Purity |
>95%, as determined by SDS-PAGE and visualized by Coomassie Brilliant Blue |
| Biol. activity |
The ED50 of the EGF receptor-Fc chimera is typically 60 -100 ng/ml as measured by its ability to neutralize EGF mediated proliferation of murine NIH3T3 fribroblasts. |
| Form |
The EGF receptor-Fc chimera consists of 5-30% carbohydrate by weight. When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1%
human serum albumin (HSA) and 10% trehalose. |
| Reconstitution |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term
storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended. |
