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Human Interleukin 1 beta ELISA Kit (2 plates)

$797.00USD


Catalogue No. BEK-2158-2P
  Interested in a 1-plate kit? email us at sales@biosensis.com to get a quote
Description The human Interleukin 1 beta (IL-1b) Kit is a sandwich ELISA. The capture antibody is a monoclonal human IL-1b antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IL-1b concentration are added to these wells and allowed to complex with the bound IL-1b antibody. A biotinylated human IL-1b monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1b present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IL-1b in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Batch No. See product labels
Other Names Interleukin-1 beta; IL-1 beta; Catabolin; IL1B; IL1F2; IL1-beta;
Accession P01584 IL1B_HUMAN;
Specificity Human IL-1b
Storage Store at 4C
Kit components The ELISA kit box contains 2 x 96 pre-coated strip plates, protein standards, detection reagents, substrate buffer and precise instructions.
Range 1.56 pg/ml - 100 pg/ml
Sensitivity < 0.15 pg/ml
Kit protocol Click to download the kit protocolBEK-2158-2P_as_at_Feb2011.pdf
Images (click to zoom)
Human Interleukin 1 beta ELISA Kit (2 plates) This standard curve is for demonstration purposes only. A standard curve should be generated for each assay.

PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

MSDS

Click to download the MSDS - Click to download the MSDS

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