| Catalogue No.: |
PE-1247-25 |
| Batch No.: |
See product label |
| Unit size: |
25 µg |
| Other Names: |
Lymph node homing receptor; Leukocyte adhesion molecule 1; LAM-1; Leukocyte surface antigen Leu-8; TQ1; gp90-MEL; Leukocyte-endothelial cell adhesion molecule 1; LECAM1; CD62L; SELL; LNHR; LYAM1; |
| Accession: |
P14151 LYAM1_HUMAN; |
| Produced in: |
A DNA sequence encoding the signal peptide, pro-peptide and extracellular domain of
human L-Selectin (amino acids 1-343) was fused to the Fc region of human IgG1 (amino acids 93-330). The chimeric protein was expressed in modified human 293 cells. |
| Description: |
L-selection (CD26L) is a cell surface adhesion protein constitutively expressed on all classes of human leukocytes. The L-Selectin glycoprotein is composed of a C-type lectin family domain at the amino terminus, an EGF-like domain, two short consensus repeat (SCR) sequences, a transmembrane domain and a short cytoplasmic tail. L-Selectin contains 7 potential N- linked glycosylation sites. |
| MW: |
The L-Selectin-Fc chimera migrates as a broad band between 75 and 105 kDa
in SDS-PAGE due to post-translation modifications, in particular glycosylation. The unmodified L-Selectin-Fc chimera has a predicted molecular mass of 59.9 kDa.
The L-Selectin-Fc chimera separates into a number of isoforms with a pI
between 4.6 and 6.5 in 2D PAGE. The unmodified L-Selectin-Fc chimera has a predicted pI of 5.92. |
| Purity: |
>95%, as determined by SDS-PAGE and visualized by silver stain |
| Form: |
The L-Selectin-Fc chimera consists of 20-45% carbohydrate by weight. |
| Reconstitution: |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage: |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 4°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended. |
