| Catalogue No.: |
PE-1238-25 |
| Batch No.: |
See product label |
| Unit size: |
25 µg |
| Other Names: |
Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; p75NTR; CD271; |
| Accession: |
P08138 TNR16_HUMAN; |
| Produced in: |
A DNA sequence encoding the signal peptide and extracellular domain of human NGF receptor (amino acids 1-250) was fused to the Fc region of human IgG1 (amino acids 93-330). The chimeric protein was expressed in modified human 293 cells. |
| Description: |
Nerve growth factor (NGF) receptor, also known as p75NTR, is a low affinity NGF receptor. It binds with equal affinity neurotrophins such as beta NGF, BDNF, NT-3 and NT-4. NGF receptors mediate signaling of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. These receptors are also thought to play a role in neurogenerative diseases such as Alzheimers disease. The NGF receptor is a type I transmembrane glycoprotein (399 aa) consisting of a signal peptide (28 aa), an extracellular domain (222 aa) which contains four cysteine rich domains responsible for ligand binding, a transmembrane domain (22 aa) and a cytoplasmic domain (155 aa). |
| MW: |
The NGF Receptor-Fc chimera migrates as a broad band between 75 and 100 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. The unmodified NGF Receptor-Fc chimera has a predicted mass of of 50.8 kDa.
The NGF Receptor-Fc chimera separates into a number of isoforms with a pI
between 4.0 and 6.0 in 2D PAGE. The unmodified NGF Receptor-Fc chimera has a predicted pI of 4.89. |
| Purity: |
>95%, as determined by SDS-PAGE and visualized by silver stain |
| Biol. activity: |
The ED50 of the NGF Receptor-Fc chimera is typically 0.7 - 1.0 ug/ml as measured by its ability to neutralize beta NGF mediated proliferation of the human growth-
factor dependent TF-1 cell line. |
| Form: |
The NGF Receptor-Fc chimera consists of 30-50% carbohydrate by weight. When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1%
human serum albumin (HSA) and 10% trehalose. |
| Reconstitution: |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage: |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term
storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C.
Repeated freeze thawing is not recommended. |
