| Catalogue No. |
PE-1242-2 |
| Description |
Stem Cell Factor (SCF) is a hematopoietic growth factor that stimulates the proliferation of mast cells. SCF also mediates cell-cell adhesion and acts synergistically with other cytokines. The actions of SCF are exerted by binding to the SCF Receptor (PE-1243-50) which results in receptor dimerization. SCF is a type I membrane glycoprotein with 5 potential N-linked glycosylation sites. Soluble SCF is produced by proteolytic cleavage of the extracellular domain. |
| Batch No. |
See product label |
| Unit size |
2 µg |
| Other Names |
Kit ligand; C-kit ligand; Stem cell factor; SCF; Mast cell growth factor; MGF; KITLG; MGF; SCF; |
| Accession |
P21583 SCF_HUMAN; |
| Produced in |
A DNA sequence encoding the signal peptide and the mature Stem Cell Factor (SCF) protein of human SCF was expressed in modified human 293 cells. |
| MW |
SCF migrates as a broad band between 20 and 42 kDa in SDS-PAGE due
to post-translational modifications. Unmodified SCF has a predicted molecular mass of 21.0 kDa.
SCF separates into a number of isoforms with a pI between 3.0 and 7.0 in
2D PAGE due to post-translational modifications. This compares with the unmodified SCF with a predicted pI of 5.76. |
| Purity |
>95%, as determined by SDS-PAGE and visualized by silver stain |
| Biol. activity |
The ED50 of SCF is typically 10-20 ng/ml as measured in a cell proliferation assay
using the human growth factor-dependent M-07e cell line. |
| Form |
Purified SCF consists of 0-50% carbohydrate by weight. |
| Reconstitution |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage |
Lyophilized products should be stored at 2-8°C. Following reconstitution, short-term storage at 4°C is recommended and longer-term storage of aliquots at -18 to -20°C.
Repeated freeze thawing is not recommended. |
