| Catalogue No. |
PE-1243-25 |
| Description |
Stem Cell Factor Receptor (SCFR) is a member of the receptor tyrosine kinase family. Binding of SCFR to its ligand Stem Cell Factor (PE-1242-5) induces receptor dimerization, autophosphorylation and signal transduction. SCFR is essential for the development of normal hematopoietic cells and plays an important role in the survival, proliferation and differentiation of mast cells, melanocytes and germ cells. |
| Batch No. |
See product label |
| Unit size |
25 µg |
| Other Names |
Mast/stem cell growth factor receptor; SCFR; Proto-oncogene tyrosine-protein kinase Kit; c-kit; CD117 |
| Accession |
P10721 KIT_HUMAN; |
| Produced in |
A DNA sequence encoding the signal peptide and extracellular domain of human Stem Cell Factor Receptor (amino acids 1-520) was expressed in modified human 293 cells. |
| Produced in |
Human |
| MW |
Under reducing conditions SCFR migrates as a broad band between 65 and 100 kDa on SDS-PAGE due to post-translational modifications. The unmodified SCFR polypeptide has a predicted monomeric molecular mass of 55.9 kDa.
SCFR separates into a number of glycoforms with a pI between 4 and 7 on 2D PAGE due to post-translational modifications. The unmodified SCFR has a predicted pI of 6.1. |
| Purity |
>95%, as determined by SDS-PAGE, visualised by Coomassie Brilliant Blue |
| Biol. activity |
The ED50 of SCFR is typically 2-4 ug/ml from its ability to neutralise SCF mediated
proliferation of the human growth dependent M-07e cell line. |
| Form |
Purified SCFR consists of approximately 15-45% carbohydrate by weight. |
| Reconstitution |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 4°C and longer-term storage of aliquots at -18 to -20°C is recommended. Repeated freeze thawing is not recommended. |
