| Catalogue No. |
PE-1235-20 |
| Description |
Tyrosine Kinase Receptor B (TrkB) is a high affinity receptor for brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5. It also binds neurotrophin 3 (NT-3) with lower affinity. TrkB does not bind nerve growth factor (NGF). TrkB is involved in the development and/or maintenance of the nervous system and is widely expressed, predominantly in nervous tissue. TrkB is a heavily glycosylated type I transmembrane protein. The TrkB-Fc chimera contains 13 potential N-linked glycosylation sites. |
| Batch No. |
See product label |
| Unit size |
20 µg |
| Other Names |
Tropomyosin-related kinase receptor, BDNF/NT-3 growth factors receptor, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, GP145-TrkB/GP95-TrkB, Trk-B, Ntrk2, Trkb, TrkB-Fc Chimera |
| Accession |
Q16620 NTRK2_HUMAN; |
| Produced in |
A DNA sequence encoding the signal peptide and extracellular domain of human TrkB (amino acids 1- 428) was fused to the Fc region of human IgG1 (amino acids 93-330). The chimeric protein was expressed in modified human 293 cells. |
| MW |
The TrkB-Fc chimera migrates as a broad band between 90 and 115 kDa in SDS-
PAGE due to post-translational modifications, in particular glycosylation. The unmodified TrkB-Fc chimera has a predicted molecular mass of 71.0 kDa.
The modified TrkB-Fc chimera separates into a number of isoforms with a pI between 4.9 and 6.7 in 2D PAGE whereas the unmodified TrkB-Fc chimera has a predicted pI of 5.79. |
| Purity |
>95%, as determined by SDS-PAGE and visualized by silver stain. |
| Applications |
The TrkB-Fc chimera bound to protein A sepharose beads is able to pull down its ligand BDNF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Form |
The chimera consists of 20-40% carbohydrate by weight. |
| Reconstitution |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 4°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended. |
