Human TrkB-Fc Chimera expressed in mammalian cells

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Home » Catalog » Proteins » Receptor Body Chimeras » PE-1235-25

Human TrkB-Fc Chimera expressed in mammalian cells

$197.00USD

Catalogue No.:	PE-1235-25
Batch No.:	See product label
Unit size:	25 µg
Other Names:	Tropomyosin-related kinase receptor, BDNF/NT-3 growth factors receptor, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, GP145-TrkB/GP95-TrkB, Trk-B, Ntrk2, Trkb, TrkB-Fc Chimera
Accession:	Q16620 NTRK2_HUMAN;
Produced in:	A DNA sequence encoding the signal peptide and extracellular domain of human TrkB (amino acids 1- 428) was fused to the Fc region of human IgG1 (amino acids 93-330). The chimeric protein was expressed in modified human 293 cells.
Description:	Tyrosine Kinase Receptor B (TrkB) is a high affinity receptor for brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5. It also binds neurotrophin 3 (NT-3) with lower affinity. TrkB does not bind nerve growth factor (NGF). TrkB is involved in the development and/or maintenance of the nervous system and is widely expressed, predominantly in nervous tissue. TrkB is a heavily glycosylated type I transmembrane protein. The TrkB-Fc chimera contains 13 potential N-linked glycosylation sites.
MW:	The TrkB-Fc chimera migrates as a broad band between 90 and 115 kDa in SDS- PAGE due to post-translational modifications, in particular glycosylation. The unmodified TrkB-Fc chimera has a predicted molecular mass of 71.0 kDa. The modified TrkB-Fc chimera separates into a number of isoforms with a pI between 4.9 and 6.7 in 2D PAGE whereas the unmodified TrkB-Fc chimera has a predicted pI of 5.79.
Purity:	>95%, as determined by SDS-PAGE and visualized by silver stain.
Applications:	The TrkB-Fc chimera bound to protein A sepharose beads is able to pull down its ligand BDNF.
Form:	The chimera consists of 20-40% carbohydrate by weight. When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution:	It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Storage:	Lyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 4°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

Images (click to zoom)

Human TrkB-Fc Chimera expressed in mammalian cells

Gel analysis of TrkB-Fc Chimera.

Lane 1: MW marker
Lane 2: TrkB-Fc Chimera (10 µg)
Lane 3: TrkB-Fc Chimera (10 µg) treated with PNGase F. Band at 35 kDa is PNGase F.

PDF Data Sheet	- Click to download the Data Sheet
MSDS	- Click to download the Material Safety Data Sheet

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