| Catalogue No. |
PE-1236-50 |
| Description |
TrkC is a member of the tropomyosin-related kinase receptor family. TrkC is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC is the receptor for neurotrophin-3 (NT-3). Signalling through TrkC leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. TrkC is a heavily glycosylated molecule with 13 potential N-linked glycosylation sites within the extracellular domain. |
| Batch No. |
See product label |
| Unit size |
50 µg |
| Other Names |
Tropomyosin-related kinase receptor; NT-3 growth factor receptor; Neurotrophic tyrosine kinase receptor type 3; TrkC tyrosine kinase; GP145-TrkC; Trk-C; NTRK3; TRKC; TrkC-Fc Chimera |
| Accession |
Q16288 NTRK3_HUMAN; |
| Produced in |
A DNA sequence encoding the signal peptide and extracellular domain of human TrkC (amino acids 1-428) was fused to the Fc region of human IgG1 (amino acids 93-330). The chimeric protein was expressed in modified human 293 cells. |
| Produced in |
Human |
| MW |
The TrkC- Fc chimera migrates as a broad band between 85 and 150 kDa in
SDS-PAGE due to post-translational modifications, in particular glycosylation. The unmodified TrkC–Fc chimera has a predicted molecular mass of 71.9 kDa.
The TrkC-Fc chimera separates into a number of isoforms with a pI between 4
and 7 in 2D PAGE. The unmodified TrkC–Fc chimera has a predicted pI of 5.8. |
| Purity |
>95%, as determined by SDS-PAGE and visualized by silver stain. |
| Form |
The TrkC-Fc chimera consists of 15-55% carbohydrate by weight. When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term
storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C.
Repeated freeze thawing is not recommended. |
