Biosensis offers a large variety of established and new, well-characterized, tracing reagents and kits for detecting degenerating neurones, myelin, amyloid plaques, and cellular sub-structures.
The Fluoro-Jade C Ready-to-Dilute Staining Kit: Fluoro Jade C stains all degenerating neurons regardless of specific insult or mechanism of cell death and exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes the Fluoro Jade kit ideal for localising degenerating nerve cell bodies as well as distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols. The Fluoro-Jade C kit is also available in a cost-effective trial size.
The Black-Gold II Ready-to-Dilute Staining Kit with Toluidine Blue O Counter Stain: This Black Gold kit has been specifically designed to identify normal and pathogenic Myelin. The Black Gold kit allows you to localise myelin, both individual fibres and tracts, along with the option of co-localising cell bodies via the Toluidine Blue counter stain. Black Gold II labelled myelinated fibres appear nearly black while the Toluidine Blue O labelled cellular Nissl bodies are blue under bright field illumination.
The Amylo-Glo Ready-to-Dilute Staining kit: This Amylo-Glo kit has been designed to identify Amyloid Plaques. This novel marker has several advantages over other conventional markers such as Thioflavin S and Congo Red because of its unique chemical and spectral properties. Amylo-Glo® RTD™ is compatible with fresh, frozen, and formalin-fixed immunohistochemistry or cytochemistry.
Recent advances in new technologies for imaging sub-cellular structures have resulted in a new class of compounds with superior characteristics for fluorescence imaging of cells and tissues. These new imaging reagents demonstrate excellent photostability, low cytotoxicity, large Stokes Shift, rapid cellular uptake, and can be used on live and fixed samples. Imaging of polar lipids (TR-600-PL), endoplasmic reticulum (TR-601-ER), mitochondria (TR-602-MIT), polar lipid droplets & endoplasmic reticulum (TR-603-LER) is specific and fast. TR-604-DYE is a luminiscent tag suitable for copper facilitated 'click' conjugation reactions.