Parallelism, linearity-of-dilution and spike-and-recovery experiments are essential validation studies that assess the ability of the ELISA assay to measure the true (ie. accurate) amount of antigen in the sample.
Acid-extraction can be used as an alternative tissue extraction method to release BDNF, which can be bound to its receptors and chaperons within many tissues. Samples prepared following this protocol can be assayed for BDNF content by ELISA and also used in Western Blotting to assess BDNF protein expression.
Need a hand in calculating antigen amount in tissue homogenates or cell lysates? Make sense of your ELISA data and get your results publication-ready with this simple Excel spreadsheet-based calculator.