| Catalogue No.: |
M-996-100 |
| Batch No.: |
See product label |
| Unit size: |
100 µg |
| Antigen: |
A synthetic peptide of 10 amino acids corresponding to the N-terminus of alpha smooth muscle actin. This antibody reacts only with alpha smooth muscle actin and does not react with actin from striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial). It also does not react with beta and gamma actins. |
| Other Names: |
ACTA2; ACTSA; ACTVS; |
| Accession: |
P62736 ACTA_HUMAN |
| Description: |
Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. Three main groups of actin isoforms have been identified in vertebrates; alpha, beta and gamma. Alpha actins are found mainly in muscle tissues. |
| Produced in: |
Mouse |
| Purity: |
IgG |
| Applications: |
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 2.0 µg/ml is recommended for WB. Human smooth muscle actin has a predicted length of 377 residues and a MW of 42 kDa. A concentration of 0.3-1.2 µg/ml is recommended to detect alpha smooth muscle actin in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. |
| Specificity: |
The specificity of this antibody has been confirmed by WB and IHC against the antigen. |
| Cross-reactivity: |
Human; mouse; pig; baboon; rabbit; |
| Blast it: |
If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example. |
| Form: |
Lyophilized from 1.2% sodium acetate with 2mg BSA and 0.2mg NaN3 added as preservative. |
| Reconstitution: |
Reconstitute in 1 ml of PBS to achieve an antibody concentration of 100 µg/ml. Centrifuge to remove any insoluble material. |
| Storage: |
At least 12 months after purchase at 2 - 4°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. |
| Expiry Date: |
12 months after purchase. |
