Mouse monoclonal antibody to alpha Synuclein [3H9]

$287.00USD

Catalogue No.	M-1402-100
Description	Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. FUNCTION: May be involved in the regulation of dopamine release and transport. Soluble protein, normally localized primarily at the presynaptic region of axons, which can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation. TISSUE SPECIFICITY: Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.SUBUNIT: Soluble monomer which can form filamentous aggregates. Interacts with UCHL1. Interacts with phospholipase D and histones. SUBCELLULAR LOCATION: Cytoplasm. Membrane. Nucleus. Note=Membrane-bound in dopaminergic neurons. Also found in the nucleus. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. Additional isoforms seem to exist.
Batch No.	See product label
Unit size	100 µl
Antigen	Recombinant full length human alpha synuclein expressed and purified from E. coli
Antibody Type	Monoclonal
Isotype	IgG1
Clone	3H9
Other Names	Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP
Accession	P37840 SYUA_HUMAN;
Produced in	Mouse
Applications	Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:1,000 - 1:5,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity	The specificity of this antibody has been confirmed by WB. This antibody detects ~15 kDa alpha synuclein protein.
Antibody Against	alpha Synuclein
Cross-reactivity	Human and Rodent. Predicted to react with other mammalian tissue.
Blast URL	Click here
Form	Lyophilised with 5% trehalose
Appearance	White powder
Reconstitution	Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage	After reconstitution of lyophilised antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date	12 months after purchase

Images (click to zoom)

Mixed neuron-glial cultures stained with Mouse monoclonal antibody to alpha Synuclein [3H9] M-1402-100 (red) and Chicken polylclonal antibody to MAP2 C-1382-50 (green). The alpha Synuclein antibody stains vesicular structures the perikarya and processes of the neurons in this image.

Western Blot image with Mouse monoclonal antibody to alpha Synuclein [3H9] M-1402-100 on recombinant alpha-synuclein (left lane) and crude extract of mouse brain (right lane). Image courtesy Rogan Tinsley, Howard Florey Institute, University of Melbourne).

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