| Catalogue No. |
M-997-100 |
| Description |
Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. Three main groups of actin isoforms have been identified in vertebrates; alpha, beta and gamma. Beta actins coexist with gamma actins in most cell types as components of the cytoskeleton and as mediators of internal cell motility. |
| Batch No. |
See product label |
| Unit size |
100 µg |
| Antigen |
A modified synthetic peptide (C-DDIALVIDNGSGK) corresponding to the N-terminus of beta Actin conjugated to KLH. This antibody reacts only with beta actin and not with alpha and gamma actins. |
| Clone |
AC-2 |
| Other Names |
ACTB; AX; actin; Actx; |
| Accession |
P60709 ACTB_HUMAN |
| Produced in |
Mouse |
| Purity |
IgG |
| Applications |
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/ml is recommended for WB. Human beta actin has a predicted length of 375 residues and the MW of the monomer is 42 kDa. A concentration of 0.2-0.5 µg/ml is recommended to detect beta actin in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
The specificity of this antibody has been confirmed by WB and IHC against the antigen. |
| Cross-reactivity |
Human; mouse; rat; |
| Blast it |
If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example. |
| Form |
Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3 |
| Reconstitution |
Reconstitute in 1 ml of PBS (pH 7.4) to achieve an antibody concentration of 100 µg/ml. Centrifuge to remove any insoluble material. |
| Storage |
At least 12 months after purchase at 2 - 4°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. |
| Expiry Date |
12 months after purchase. |
