| Catalogue No. |
M-995-100 |
| Description |
Assembly Polypeptides (AP), also known as adaptors, co-assemble with clathrin during the formation of clathrin coated membranes. AP2 adaptors are associated with the endocytic clathrin coated vesicles at the plasma membrane. The AP2 adaptor is composed of four subunits which includes alpha- and beta-adaptin. This antibody reacts specifically with the alpha-adaptin subunit of the AP2 adaptor. |
| Batch No. |
See product label |
| Unit size |
100µg |
| Antigen |
Assembly polypeptide 2 (AP2) adaptor polypeptides from bovine brain |
| Clone |
Ada-1 |
| Other Names |
AP2A1;AP-2; |
| Accession |
O95782 AP2A1_HUMAN; Q1JPJ7 Q1JPJ7_BOVIN |
| Produced in |
Mouse |
| Purity |
IgG |
| Applications |
Western Blotting (WB). A concentration of 2.0-4.0 µg/ml is recommended for WB. Human alpha adaptin (isoform #1) has a predicted length of 977 residues and MW of 108 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
The specificity of this antibody has been confirmed by WB against the antigen. |
| Cross-reactivity |
Human; rat; |
| Blast it |
If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example. |
| Form |
Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3 |
| Reconstitution |
Reconstitute in 1 ml of PBS (pH 7.4) to achieve an antibody concentration of 100 µg/ml. Centrifuge to remove any insoluble material. |
| Storage |
At least 12 months after purchase at 2 - 4°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. |
| Expiry Date |
12 months after purchase |
