| Catalogue No. |
M-1173-100 |
| Description |
Cyclin-dependent kinase 4 (CDK4) belongs to the Ser/Thr protein kinase family. CDK4 is a subunit of the protein kinase complex that is important for cell cycle G1 phase progression. Mutations in CDK4 have been implicated in tumor formation. Multiple alternatively spliced variants and polyadenylation sites have been reported for this gene. |
| Batch No. |
See product label |
| Unit size |
100 µg |
| Antigen |
Recombinant human Cyclin-dependent kinase 4 (CDK4) |
| Other Names |
Cell division protein kinase 4; PSK-J3; CDK4; |
| Accession |
P11802 CDK4_HUMAN; |
| Produced in |
Mouse |
| Purity |
IgG |
| Applications |
Western Blotting (WB). A concentration of 0.5-1.0 µg/ml is recommended for WB. Human CDK4 has a predicted length of 303 residues and MW of 34 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
The specificity of this antibody has been confirmed by WB against the antigen. |
| Cross-reactivity |
Human; mouse; rat; |
| Blast it |
If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example. |
| Form |
Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3 |
| Reconstitution |
Reconstitute in 1 ml of PBS (pH 7.4) to achieve an antibody concentration of 100 µg/ml. Centrifuge to remove any insoluble material. |
| Storage |
At least 12 months after purchase at 2 - 4°C (lyophilized formulations).
After reconstitution, aliquot and store at -20°C for a higher stability.
Avoid freeze-thaw cycles |
| Expiry Date |
12 months after purchase |
