| Catalogue No.: |
M-881-100 |
| Batch No.: |
See product label |
| Unit size: |
100 µg |
| Antigen: |
Partial recombinant human APG5 (176-275) with a GST tag. |
| Other Names: |
Autophagy protein 5, APG5-like, Apoptosis -specific protein, GENE NAMEs: ATG5, APG5L, ASP, APG5 |
| Accession: |
ATG5_HUMAN |
| Description: |
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. APG5 conjugates to APG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. It localise to the cytoplasm and it co-localise with nonmuscle actin. APG5 is ubiquitously expressed. Its expression is dramatically increased in apoptotic cells. Conjugation to APG12 is essential for autophage but it is not required for association with isolation membrane. |
| Produced in: |
Mouse |
| Purity: |
Protein G purified immunoglobulin |
| Applications: |
This antibody is recommended for WB, and sandwich ELISA. |
| Specificity: |
Specificity has been confirmed by WB and direct ELISA against the antigen. |
| Cross-reactivity: |
Human. Other species have not been tested. |
| Blast it: |
To see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence that you are interested in and copy and paste it HERE and blast/format it. |
| Form: |
Lyophilised mouse IgG from PBS, pH 7.2. |
| Reconstitution: |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage: |
After reconstitution keep aliquots at -20ºC for higher stability or at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date: |
12 months after purchase |
