| Catalogue No. |
M-879-100 |
| Description |
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy. ATG12 is conjugated to ATG5. The ATG12-ATG5 conjugate has an apparent molecular mass of 65 kDa. The C-terminal glycine of ATG12 is conjugated to a central lysine (lys130) of ATG5. Studies showed that over expression of ATG3 facilitated formation of the ATG12-ATG5 conjugate, suggesting that ATG3 cross-talks with the ATG12 conjugation system. ATG12 is ubiquitously expressed.
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| Batch No. |
See product label |
| Unit size |
100 µg |
| Antigen |
Full recombinant human ATG12, isoform 2, (1-74) with a GST tag. |
| Clone |
2H8 |
| Other Names |
Autophagy-related protein 12; ATG12; APG12; APG12L; APG12-like; |
| Accession |
ATG12_HUMAN |
| Produced in |
Mouse |
| Purity |
Protein G purified immunoglobulin |
| Applications |
This antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
Specificity has been confirmed by WB and direct ELISA against the antigen. |
| Cross-reactivity |
Human. Other species have not been tested. |
| Blast it |
To see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence that you are interested in and copy and paste it HERE and blast/format it. |
| Form |
Lyophilised from PBS pH 7.2 |
| Reconstitution |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution keep aliquots at -20ºC for higher stability or at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date |
12 months after purchase |
