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Mouse monoclonal antibody to human Ena/VASP-like protein [5G1]: IgG

$287.00USD


Catalogue No. M-898-100
Description
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. Ena/VASP-like protein enhances actin nucleation ad polymerisation. It is required to transform actin polymerisation into active movement for the propulsive force of Listeria monocytogenes. It exists as a homotetramerwhich localises to the cytoplasm, cytoskeleton and cell projection. There are two name isoforms produced by alternative splicing.
Batch No. See product label
Unit size 100 µg
Antigen Partial recombinant human Ena/VASP-like protein (309-419) with a GST tag.
Clone 5G1
Other Names Ena/vasodilator-stimulated phosphoprotein-like; EVL; RNB6
Accession EVL_HUMAN
Produced in Mouse
Purity Protein G purified immunoglobulin
Applications This antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Specificity has been confirmed by WB and direct ELISA against the antigen.
Cross-reactivity Human. Other species have not been tested.
Blast it To see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence that you are interested in and copy and paste it HERE and blast/format it.
Form Lyophilised from PBS pH 7.2
Reconstitution Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20ºC for higher stability or at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Mouse monoclonal antibody to human Ena/VASP-like protein [5G1]: IgG
Western blot detection of Ena/VASP-like protein expression in Transfected 293T cell lysate. This antibody detects a band of molecular weight 45 kDa in Ena/VASP-like protein transfected lysate (lane 1) and is negative against non-transfected 293T lysate.
Mouse monoclonal antibody to human Ena/VASP-like protein [5G1]: IgG Sandwich ELISA using anti-Ena/VASP-like protein monoclonal antibody as a capture antibody. The detection limit for GST tagged recombinant antigen is 0.03 ng/ml.
PDF Data Sheet

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MSDS

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