Mouse monoclonal antibody to human Laminin [LAM-26]: IgG

$287.00USD

Catalogue No.	M-982-100
Description	Laminin is a large (900 kDa) complex extracellular glycoprotein and a major component of the basement membrane. Laminin is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin is composed of three different polypeptide chains; alpha, beta and gamma which are bound to each other by disulphide bonds into a cross-shaped molecule. Isoforms have been detected for all three chains. The different combinations of the alpha, beta and gamma chains give rise to at least 15 laminin heterotrimers.
Batch No.	See product label
Unit size	100 µg
Antigen	Human laminin
Clone	LAM-26
Other Names	LAMA; LAMA1;
Accession	P25391 LAMA1_HUMAN
Produced in	Mouse
Purity	IgG
Applications	Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.25-0.5 µg/ml is recommended for WB. Human laminin (subunit alpha-1) has a predicted length of 3,075 residues and a MW of 337 kDa. A concentration of 0.5-1.0 µg/ml is recommended to detect laminin in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity	The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Cross-reactivity	Human; pig; feline;
Blast it	If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form	Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3
Reconstitution	Reconstitute in 1 ml of PBS (pH 7.4) to achieve an antibody concentration of 100 µg/ml. Centrifuge to remove any insoluble material.
Storage	At least 12 months after purchase at 2 - 4°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date	12 months after purchase

PDF Data Sheet	- Click to download the Data Sheet
MSDS	- Click to download the MSDS