| Catalogue No. |
M-967-100 |
| Description |
Bcl-10 is a 26 kDa (233 aa) protein expressed in many human and murine tissues. It contains an N-terminal caspase recruiting domain and is involved in the activation of caspase9 to induce apoptosis. |
| Batch No. |
See product label |
| Unit size |
100 µg |
| Antigen |
Recombinant full-length human Bcl-10. This antibody reacts with the epitope within the region 122-168 from human Bcl 10. |
| Other Names |
CIPER; CLAP; CARMEN; mE10; Bcl10; |
| Accession |
O95999 BCL10_HUMAN |
| Produced in |
Mouse |
| Purity |
IgG |
| Applications |
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/ml is recommended for WB. Human Bcl-10 has a predicted length of 233 aa and MW of 26 kDa. A concentration of 2.0-4.0 µg/ml is recommended to detect Bcl-10 in formalin fixed and paraffin embedded tissues. Antigen retrieval may improve the staining. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Follicular and diffuse large B cell lymphoma tumours were used to test specificity. Also the lymphoid organs and cytoplasm of mammary gland cells from normal tissues were used to test the specificity of this antibody in human tissue. |
| Cross-reactivity |
Human |
| Blast it |
If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example. |
| Form |
Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3 |
| Reconstitution |
Reconstitute in 1 ml of PBS (pH 7.4) to achieve an antibody concentration of 100 µg/ml. Centrifuge to remove any insoluble material. |
| Storage |
At least 12 months after purchase at 2 - 4°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. |
| Expiry Date |
12 months after purchase. |
