| Catalogue No. |
M-968-100 |
| Description |
Apoptosis regulator Bcl-2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. At least 2 isoforms (alpha and beta) are produced by alternate splicing with different C-terminal ends. |
| Batch No. |
See product label. |
| Unit size |
100 µg |
| Antigen |
Synthetic peptide (GAAPAPGIFSSQPG) corresponding to amino acids 41-54 from human Bcl-2 conjugated to thyroglobin. |
| Other Names |
BCL2; |
| Accession |
P10415 BCL2_HUMAN |
| Produced in |
Mouse |
| Purity |
IgG |
| Applications |
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/ml is recommended for WB. Human Bcl-2 has a predicted length of 239 aa and MW of 26 kDa. A concentration of 0.4-1.0 µg/ml is recommended to detect Bcl-2 in formalin fixed and paraffin embedded tissues as well as formalin/acetone frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human breast cancer cell line MCF-7, Hela cells, normal and reactive lymphoid tissues were used to test the specificity of this antibody. |
| Cross-reactivity |
Human; Rat; |
| Blast it |
If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example. |
| Form |
Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3 |
| Reconstitution |
Reconstitute in 1 ml of PBS (pH 7.4) to achieve an antibody concentration of 100 µg/ml. Centrifuge to remove any insoluble material. |
| Storage |
At least 12 months after purchase at 2 - 4°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. |
| Expiry Date |
12 months after purchase. |
