Mouse monoclonal antibody to human p75NTR [ME20.4]

Mouse monoclonal antibody to human p75NTR [ME20.4] - FITC

$245.00USD

Catalogue No.:	M-017-50-FT
Batch No.:	See product label
Unit size:	50 µg
Antigen:	The p75NTR antibody was derived from immunization of mice with human WM245 melanoma cells.
Other Names:	Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR
Accession:	TNR16_HUMAN
Description:	Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) due to its molecular mass and its ability to bind at low affinity not only NGF (see 162030), but also other neurotrophins, including brain-derived neurotrophic factor (BDNF; 113505), neurotrophin-3 (NTF3; 162660), and neurotrophin-4/5 (NTF5; 162662). At the time of its discovery, NGFR was considered a unique type of protein. Subsequently, however, a large superfamily of tumor necrosis factor receptors were found to share the overall structure of NGFR (4 extracellular ligand-binding, cysteine-rich repeats, or CRs, and signaling through association with, or disassociation from, cytoplasmic interactors). The identification of this superfamily helped elucidate some of the biologic functions of NGFR, including its ultimate involvement in the nuclear factor kappa-B (NFKB; see 164011) and apoptosis pathways. As a monomer, NGFR binds NGF with low affinity. Higher affinity binding is achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA (NTRK1; 191315), TRKB (NTRK2; 600456), and TRKC (NTRK3; 191316). TRKA, TRKB, and TRKC are specific for or 'preferred by' NGF, NTF5 and BDNF, and NTF3, respectively. NTF3 also binds to TRKA and TRKB, but with significantly lower affinity.
Produced in:	P75 knock-out mouse.
Purity:	Immunoglobulin (IgG2A) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The antibody was then conjugated to Fluorescein isomer 1 (FITC, Sigma). A minimum fluorescein: protein ratio of 3:1 is guaranteed. The conjugate was purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
Applications:	This antibody is reccommended for use in immunohistochemistry, immunofluorescence, flow cytometry and NGF receptor p75 dynamics. For immunohistochemistry a concentration of 2 µg/ml is recommended. Not appropriate for Western Blots. For FACS a concentration of 20 µg/ml is recommended and for 1 site ELISA at least a 1 in 5000 diltution.This antibody does not block Neurotrophin binding. Optimal working dilutions should be determined by the end user.
Specificity:	This antibody recognises p75NTR (low affinity neurotrophin receptor)
Cross-react:	Reacts with rat, human, cat, dog, pig, rabbit and sheep. Does not react with mouse.
Form:	Liquid, in 10mM Tris, 50mM NaCl solution. Centrifuge to remove any insoluble material. No preservatives added.
Storage:	The antibody conjugate can be stored at 4ºC for up to 4 months with the addition of appropriate antibacterial agent.
Expiry Date:	Four months after purchase
References:	1.Walker BR et al. (2007) Behav Brain Res 176:109-20
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