Mouse monoclonal antibody to human p75NTR [MLR2]

Mouse monoclonal antibody to human p75NTR [MLR2] - ATTO 488

$287.00USD

Catalogue No.	M-028-50-AT
Description	p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR is not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor.
Batch No.	See product label
Unit size	50 µg
Antigen	Human recombinant p75.
Other Names	Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR
Accession	TNR16_HUMAN
Produced in	P75 knock-out mouse.
Purity	Immunoglobulin (IgG2A) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The IgG was then conjugated to ATTO 488 (ATTO TEC) and purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
Applications	Immunohistochemistry, immunofluorescence, WB using native p75NTR, flow cytometry (Anti NGF receptor p75 is CD271), Anti-NGF receptor p75 dynamics, retrograde transport studies, study of intracellular trafficking (see IHC image attached). Suggested working dilutions: For immunohistochemistry, a concentration of 1-2 µg/ml is recommended. For FACS, the recommended concentration is 20 µg/ml and for 1 site ELISA, at least 1 in 5000 dilution is recommended. This antibody does not block Neurotrophin binding. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity	Specificity was demonstrated by immunohistochemistry and FACS analysis. Immunohistochemical staining of motor neurons in spinal cord following lesion of sciatic nerve and in cholinergic neurons of the basal forebrain corresponding to published literature.
Cross-reactivity	Reacts with human, mouse and rat. Other species have not yet been tested but it is expected that this antibody will be useful for the study of p75 in primates and other species.
Form	Liquid, 50 µg. Centrifuge to remove any insoluble material. No preservatives added.
Storage	The antibody conjugate can be stored at 4ºC for up to 4 months with the addition of appropriate antibacterial agent.
Specific References	1. Wiese S, Herrmann T, Drepper C, Jablonka S, Funk N, Klausmeyer A, Rogers ML, Rush RA & Sendtner M (2009) Isolation and enrichment of embryonic mouse motoneurons from the lumbar spinal cord of individual mouse embryos. Nat Protoc. 2010;5(1):31-8. 2. Chacón PJ, Arévalo MA, Tébar AR. (2010) NGF-activated protein tyrosine phosphatase 1B mediates the phosphorylation and degradation of I-kappa-Balpha coupled to NF-kappa-B activation, thereby controlling dendrite morphology. Mol Cell Neurosci. 2010 Apr;43(4):384-93.
References	1. Rogers ML, Atmosukarto I, Berhanu DA, Matusica D, Macardle P, Rush RA (2006). Functional monoclonal antibodies to p75 neurotrophin receptor raised in knockout mice. J Neurosci Methods. 158(1) pp. 109-120. 2. Matusica D, Fenech MP, Rogers ML, Rush RA. (2008) Characterisation and use of the NSC-34 cell liner for study of nerurotrophin receptor trafficking. J. Neurosci. Res. 86(3) pp. 553-65. 3. Huh CY, Danik M, Manseau F, Trudeau LE, Williams S. (2008) Chronic exposure to nerve growth factor increases acetylcholine and glutamate release from cholinergic neurons of the rat medial septum and diagonal band of Boca via mechanisms mediated bu p75NTR. J. Neurosci. 28(6) pp. 1404-9. 4. Lagares A, Li HY, Zhou XF, Avendano C. (2007) Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio-neuronal precursor maturation. J. Neurosci. 27(30) pp. 7939-53.

Images (click to zoom)

Mouse monoclonal antibody to human p75NTR [MLR2] - ATTO 488

Live confocal imaging of intracellular trafficking of p75NTR using ATTO 488 labelled mouse monoclonal antibody to human p75NTR [MLR2], catalogue number M-028-50-AT in NSC34 mouse motor neuron cell line.

	Photostability of ATTO 488 compared to common dyes in water. Excitation at 488nm with 1W Argon-Ion Laser.
	Immunofluorescent staining of MLR2_Alexa488 uptake into mesenteric nerves following invivo intraperitoneal administration in adult rat.

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