Mouse monoclonal antibody to human p75NTR [MLR2]: IgG

$245.00USD

Catalogue No.:	M-009-100
Batch No.:	See product label
Unit size:	100 µg
Antigen:	Extracellular domain of human p75NTR
Other Names:	Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR
Accession:	TNR16_HUMAN
Description:	p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR is not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3.
Produced in:	Mouse
Purity:	Protein G purified immunoglobulin
Applications:	IHC, WB and Immunopanning. A concentration of 1-5 µg/ml is recommended for immunohistochemistry, immunopanning and Western Blot. A concentration of 20 µg/ml is recommended for immunofluorescence and FACS. The optimal working dilution should be determined by the end user.
Specificity:	Specificity has been confirmed using a number of techniques as described in the reference by Rogers et al (2006).
Cross-reactivity:	This antibody is known to react with human, mouse, rat and guinea-pig p75NTR protein.
Form:	Lyophilised
Reconstitution:	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage:	After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date:	12 months after purchase
References:	1. Wiese S, Herrmann T, Drepper C, Jablonka S, Funk N, Klausmeyer A, Rogers ML, Rush RA & Sendtner M (2009) Isolation and enrichment of embryonic mouse motoneurons from the lumbar spinal cord of individual mouse embryos. Nat Protoc. 2010;5(1):31-8. 2. Rogers ML, Atmosukarto I, Berhanu DA, Matusica D, Macardle P, Rush RA (2006). Functional monoclonal antibodies to p75 neurotrophin receptor raised in knockout mice. J Neurosci Methods. 158(1) pp. 109-120. 3. Matusica D, Fenech MP, Rogers ML, Rush RA. (2008) Characterisation and use of the NSC-34 cell liner for study of nerurotrophin receptor trafficking. J. Neurosci. Res. 86(3) pp. 553-65. 4. Huh CY, Danik M, Manseau F, Trudeau LE, Williams S. (2008) Chronic exposure to nerve growth factor increases acetylcholine and glutamate release from cholinergic neurons of the rat medial septum and diagonal band of Boca via mechanisms mediated bu p75NTR. J. Neurosci. 28(6) pp. 1404-9. 5. Lagares A, Li HY, Zhou XF, Avendano C. (2007) Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio-neuronal precursor maturation. J. Neurosci. 27(30) pp. 7939-53.

Images

Mouse monoclonal antibody to human p75NTR [MLR2]: IgG

Immunohistochemical staining of p75NTR in Balb/C mouse brain (septum) using mouse monoclonal antibody to human p75NTR [MLR2], catalogue number M-009-100. 4% paraformaldehyde fixed mouse brain free floating sections were incubated with mouse monoclonal antibody to human p75NTR [MLR2] (1µg/ml) overnight, followed by incubation with biotinylated Goat anti-mouse IgG conjugate at a dilution of 1: 500 and Vector ABC, DAB stained.

	Flow cytometry analysis of endogenously expressed p75NTR on ShSY5Y cells. X-63 conjugated control IgG represents negative control for all experiments.
	Flow cytometry analysis of BSR-p75 fibroblasts transfected with mouse p75NTR. X-63 conjugated control IgG represents negative control for all experiments.
	Immunofluorescent detection of p75NTR in cultured mouse NSC34 cells using a mouse monoclonal antibody to human p75NTR [MLR2], catalogue number M-009-100.
	Immunofluorescence staining of adult mouse basal forebrain cholinergic neurons with mouse monoclonal antibody to human p75NTR [MLR2], catalogue number M-009-100, with ALEXA488 secondary antibodies.

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