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Mouse monoclonal antibody to human PAFAH subunit gamma [8C11]: IgG

$287.00USD


Catalogue No. M-856-100
Description
Cytosolic PAFAH IB is formed of three subunits, alpha (45kDa), beta (30kDa, and gamma (29kDa). Its catalytic activity of PAFAH IB resides in the beta and gamma subunits while the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity. Specifically, PAFAH subunit gamma inactivates platelet-activating factor by removing the acetyl group at the sn-2 position. It plays an important role during the development of the brain. In the adult, it is expressed in brain, skeletal muscle, kidney, thymus, spleen, colon, testis, ovary and peripheral blood leukocytes. In the fetus, highest expression occurs in the brain.
Batch No. See product label
Unit size 100 µg
Antigen Partial recombinant human PAFAH subunit gamma (1-232) with a GST tag.
Other Names Platelet-activating factor acetylhydrolase IB subunit gamma, PAF acetylhydrolase 29 kDa subunit, PAF-AH 29 kDa subunit, PAF-AH subunit gamma, PAFAH subunit gamma, GENE NAMES: PAFAH1B3, PAFAHG
Accession PA1B3_HUMAN
Produced in Mouse
Purity Protein G purified immunoglobulin
Applications This antibody is recommended for WB, sandwich ELISA and immunofluorescence. The recommended dilution for this antibody is 25 µg/ml for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Specificity has been confirmed by WB and direct ELISA against the antigen.
Cross-reactivity Human and mouse. Other species havve not been tested.
Blast it To see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence that you are interested in and copy and paste it HERE and blast/format it.
Form Lyophilised mouse IgG from PBS, pH 7.2.
Reconstitution Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20ºC for higher stability or at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Mouse monoclonal antibody to human PAFAH subunit gamma [8C11]: IgG
Immunofluorescent detection of PAFAH subunit gamma expression in HeLa cells. The anti-PAFAH subunit gamma primary antibody, catalogue number M-856-100, was used at a concentration of 25 µg/ml.
Mouse monoclonal antibody to human PAFAH subunit gamma [8C11]: IgG Western blot detection of PAFAH subunit gamma expression in human neuroblastoma cell lysate.
PDF Data Sheet

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MSDS

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