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Mouse monoclonal antibody to Neurofilament Light [NF-68]: IgG

$297.00USD


Catalogue No. M-987-100
Description Neurofilaments are composed of three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins. This antibody specifically recognises dephosphorylated NF68.
Batch No. See product label
Unit size 100 g
Antigen Pig spinal cord
Clone NF-68
Other Names NF-L; NF68; NEFL; Neurofilament light polypeptide; NFL;
Accession P02547 NFL_PIG; P07196 NFL_HUMAN
Produced in Mouse
Purity IgG
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 g/ml is recommended for WB. Pig NF68 has a predicted length of 549 aa and MW of 68 kDa. A concentration of 2.0-4.0 g/ml is recommended to detect NF68 in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Cross-reactivity Human; pig;
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3
Reconstitution Reconstitute in 1 ml of PBS (pH 7.4) to achieve an antibody concentration of 100 g/ml. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 4C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase.
PDF Data Sheet

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MSDS

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