| Catalogue No. |
M-1164-100 |
| Description |
The T-cell receptor-CD3 complex (CD3-TCR) plays an important role in mediating signaling transduction. The CD3-TCR complex consists of either a TCR alpha/beta or TCR gamma/delta heterodimer co-expressed at the cell surface with the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These subunits are single-pass type I membrane proteins expressed on normal peripheral blood lymphocytes, thymocytes and Purkinje cells in the cerebellum. |
| Batch No. |
See product label |
| Unit size |
100 µg |
| Antigen |
Human thymocytes followed by Sezary T cells |
| Other Names |
T-cell surface glycoprotein CD3; CD3E; CD3G; CD3H; CD3Q; CD3Z; T cell receptor T3; |
| Accession |
P04234 CD3D_HUMAN; P07766 CD3E_HUMAN; |
| Produced in |
Mouse |
| Purity |
IgG |
| Applications |
Immunohistochemistry (IHC-Frozen). A concentration of 1.0 µg/ml is recommended to detect CD3 in formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
The specificity of this antibody has been confirmed by immunohistochemistry against the antigen. |
| Cross-reactivity |
Human; rat; |
| Blast it |
If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practice is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example. |
| Form |
Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3 |
| Reconstitution |
Reconstitute in 1 ml of PBS (pH 7.4) to achieve an antibody concentration of 100 µg/ml. Centrifuge to remove any insoluble material. |
| Storage |
At least 12 months after purchase at 2 - 4°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. |
| Expiry Date |
12 months after purchase |
