Mouse monoclonal antibody to rat p75NTR [MC192] - ATTO 488

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Home » Catalog » Antibodies » Neurotrophic Receptors » M-026-50-AT

Mouse monoclonal antibody to rat p75NTR [MC192] - ATTO 488

$245.00USD

Catalogue No.:	M-026-50-AT
Batch No.:	See product label
Unit size:	50 µg
Antigen:	Rat p75NTR
Other Names:	Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR
Accession:	TNR16_RAT
Description:	Monoclonal antibody MC192 against the rat low affinity nerve growth factor receptor (p75NTR) is derived from the fusion of Sp2/0-Ag 14 myeloma cells with mouse immune splenocytes. MC192 monoclonal antibody was originally generated by Chandlers et al. p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. MC192 has a potential use as the ligand for gene delivery into p75NTR-expressing rat cells via a receptor-mediated mechanism. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1 (By similarity).
Produced in:	P75 knock-out mouse.
Purity:	Immunoglobulin (IgG2A) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The IgG was then conjugated to ATTO 488 (ATTO TEC) and purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
Applications:	Suggested working dilutions:For immunohistochemistry a concentration of 1-2 µg/ml is recommended. The antibody is not appropriate for Western Blots. For FACS the recommended concentration is 20 µg/ml. At least 1 in 5000 dilution is recommended for 1 site ELISA.This antibody does not block Neurotrophin binding. Optimal working dilution should be determined by the end user.
Specificity:	MC192 recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat. MC192 antibody may be used for immunocytochemical localisation of rat cells expressing p75NTR, ELISA and western blot. This antibody has also been used for the construction of the MC192-saporin immunotoxin for specific elimination of neuronal populations in basal forebrain cholinergic neurons to generate an animal model for Alzheimer's disease. Using Flow Cytometry, this antibody has frequently been employed for panning to isolate p75NTR-expressing rat cells.
Cross-react:	Reacts with rat. Does not react with mouse.
Form:	Liquid, 50 µg. Centrifuge to remove any insoluble material. No preservatives added.
Storage:	The antibody conjugate can be stored at 4ºC for up to 4 months with the addition of appropriate antibacterial agent.
References:	1. Chandler, C. E. et al (1984) J Biol Chem 259, 6882-6889 2. Lagares A et al (2007) J of neurosci 27(30), 7939-7953

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Mouse monoclonal antibody to rat p75NTR [MC192] - ATTO 488

Photostability of ATTO 488 compared to common dyes in water. Excitation at 488nm with 1W Argon-Ion Laser.

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