| Catalogue No.: |
M-1217-100 |
| Batch No.: |
See product label |
| Unit size: |
100 µg |
| Antigen: |
A synthetic peptide (VDSERRPHFPQFSYSAS) corresponding to a region (461-477) from human RAC-alpha serine/threonine-protein kinase. |
| Other Names: |
Protein kinase B alpha; 2.7.11.1; RAC-PK-alpha; PKB; C-AKT; AKT1; RAC; |
| Accession: |
P31749 AKT1_HUMAN; |
| Description: |
The protein kinase AKT has a role in glucose transport, glycogen synthesis, inhibiting apoptosis, cell-cycle progression, cell growth and cancer. Three isoforms of the AKT gene have been identified; AKT1, AKT2 and AKT3. AKT1 or RAC-alpha serine/threonine-protein kinase is activated by insulin and various growth and factors. |
| Produced in: |
Mouse |
| Purity: |
IgG |
| Applications: |
Western Blotting (WB). A concentration of 0.25-0.50 µg/ml is recommended for WB. Human AKT1 has a predicted length of 480 residues and MW of 56 kDa. |
| Specificity: |
The specificity of this antibody has been confirmed by WB against the antigen. |
| Cross-reactivity: |
Human; mouse; rat; chicken; |
| Blast it: |
If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example. |
| Form: |
Lyophilized from 1.2% sodium acetate with 2mg BSA and 0.2mg NaN3 added as preservative. |
| Reconstitution: |
Reconstitute in 1 ml of PBS (pH 7.4) to achieve an antibody concentration of 100 µg/ml. Centrifuge to remove any insoluble material. |
| Storage: |
At least 12 months after purchase at 2 - 4°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. |
| Expiry Date: |
12 months after purchase. |
