| Catalogue No. |
M-1223-100 |
| Description |
Talin is a large, multidomain focal adhesion protein involved in the assembly of actin filaments. Talin interacts with the intracellular domains of integrins, vinculin and other focal adhesion proteins. |
| Batch No. |
See product label |
| Unit size |
100 µg |
| Antigen |
Talin purified from chicken gizzard |
| Clone |
Tal-33 |
| Other Names |
Talin-1; TLN1; KIAA1027; TLN; Talin-2; TLN2; KIAA0320; |
| Accession |
P54939 TLN1_CHICK; Q9Y490 TLN1_HUMAN; Q9Y4G6 TLN2_HUMAN; |
| Produced in |
Mouse |
| Purity |
IgG |
| Applications |
Western Blotting (WB). A concentration of 2.0-4.0 µg/ml is recommended to detect the protein in cells and tissues. This antibody reacts with both Talin-1 and Talin-2. It recognizes an epitope located on the intact Talin and a 190 kDa fragment. It does not recognize the 47 kDa fragment obtained by protease cleavage of chicken gizzard Talin. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
The specificity of this antibody has been confirmed by WB against the antigen. |
| Cross-reactivity |
Human; mouse; rat; chicken; frog; |
| Blast it |
If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example. |
| Form |
Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3 |
| Reconstitution |
Reconstitute in 1 ml of PBS (pH 7.4) to achieve an antibody concentration of 100 µg/ml. Centrifuge to remove any insoluble material. |
| Storage |
At least 12 months after purchase at 2 - 4°C (lyophilized formulations).
After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. |
| Expiry Date |
12 months after purchase |
