Mouse monoclonal antibody to the Tyrosine Kinase Receptor A (TrkA)

$267.00USD

Catalogue No.:	M-957-100
Batch No.:	See product label
Unit size:	100µg
Antigen:	Recombinant human TrkA expressed on 3T3 cells.
Other Names:	Tropomyosin-related kinase receptor, High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, TRK1 transforming tyrosine kinase protein, p140-TrkA, Trk-A, GENE NAME: NTRK1, TRK.
Accession:	NTRK1_HUMAN
Description:	TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Cell membrane; single-pass type I membrane protein. Endocytosed to the endosomes upon treatment of cells with NGF. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. Both isoforms have similar biological properties. TISSUE SPECIFICITY: Isoform TrkA-II is primarily expressed in neuronal cells. Isoform TrkA-I is found in non-neuronal tissues. Mutations in TrkA have been associated with congenital insensitivity to pain, anhidrosis, self-mutalating behaviour, mental retardation and cancer.
Produced in:	Mouse (Balb/c)
Purity:	Pure form, protein G purified (IgG).
Applications:	FACS, biological assays, WB (non-reducing conditions), immunohistochemistry. The optimal working dilution should be determined by the end user.
Specificity:	Specificity was demonstrated by Flow Cytometry (FACS) binding assays in NIH-3T3 cells transfected with TrkA, TrkB, TrkC and p75NTR and immunohistochemistry of normal human brain.
Cross-reactivity:	Recognizes Human TrkA only. Does not react in any other species.
Form:	Lyophilised
Reconstitution:	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage:	After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date:	12 months after purchase
References:	1. Guillemard V. and Saragovi H.U.(2001) Taxane-antibody conjugates afford potent cytotoxicity, enhanced solubility, and tumor target selectivity. Cancer Res. 2001 Jan 15;61(2):694-9. 2. Maliartchouk S. et al (2000) A designed peptidomimetic agonistic ligand of TrkA nerve growth factor receptors. Mol Pharmacol. 2000 Feb;57(2):385-91. 3. la Sala A. et al. (2000) Ligand activation of nerve growth factor receptor TrkA protects monocytes from apoptosis. J Leukoc Biol. 2000 Jul;68(1):104-10. 4. LeSauteur L. et al. (1996) Small molecule nerve growth factor analogs image receptors in vivo. Nat Biotechnol. 1996 Sep;14(9):1120-2. 5. LeSauteur L. et al. (1996) Potent human p140-TrkA agonists derived from an anti-receptor monoclonal antibody. J Neurosci. 1996 Feb 15;16(4):1308-16. 6. Kramer et al. (1997) Eur J Cancer. 33(12); pp. 2090-1. 7. Maliartchouk and Saragovi. (1997) J Neurosci. 17 (16); 6031-7. 8. Saragovi U. et al. (1998) Biol. Chem. 273(52); 34933-40.

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Mouse monoclonal antibody to the Tyrosine Kinase Receptor A (TrkA)

Immmunoreactivity of Trk A (M-957-100) in normal human brain. In the pontine nuclei, many weakly to strongly staining neurons (arrows) are seen within the fibre network (small arrows) and around the non labeled fibre bundles (f). LeSauteur L. et al, J. Neurosci. (1996)

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