| Catalogue No. |
M-958-100 |
| Description |
TrkC is a member of the neurotrophic tyrosine receptor kinase family. TrkC is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC is the receptor for neurotrophin-3 (NT-3). Signalling through TrkC leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 4 named isoforms produced by alternative splicing. Additional isoforms seem to exist. Mutations in TrkC have been associated with medulloblastomas, secretory breast carcinomas and other cancers. This antibody has been well-characterized as a selective TrkC ligand that can be used to target the human and murine TrkC receptor without p75NTR binding and activation. The antibody acts as a partial agonist to the TrkC receptor and defines a new "hot-spot" within the juxtamembrane region of the extracellular ectodomain of the receptor. Binding to this hot spot, the receptor induces survival, but not differentiation of TrkC expressing cells. Activation of the TrkC receptor is accompanied by activation of the signalling molecules, Akt and MAPK. |
| Batch No. |
See product label. |
| Unit size |
100 ”g |
| Antigen |
Immunogen was a linear recombinant human TrkC peptide conjugated to KLH. |
| Other Names |
Tropomyosin-related kinase receptor; NT-3 growth factor receptor; Neurotrophic tyrosine kinase receptor type 3; TrkC tyrosine kinase; GP145-TrkC; Trk-C; NTRK3; TRKC |
| Accession |
NTRK3_HUMAN |
| Produced in |
Mouse |
| Purity |
Pure form, protein G purified (IgG). |
| Applications |
Uses include FACS, WB (non-reducing conditions), immunohistochemistry and biological function studies (eg, activation of TrkC without p75). Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
The antibody is competitively blocked by the natural TrkC ligand, Neurotrophin 3 (NT3) but not the homologue Nerve Growth Factor (NGF) in a dose dependent manner. |
| Cross-reactivity |
Reacts with human, rat and mouse TrkC. Other species have not yet been tested. |
| Form |
Lyophilised |
| Reconstitution |
Reconstitute in 100 ”l of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution keep aliquots at -20șC for a higher stability, and at 4șC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date |
12 months after purchase |
| References |
1. Guillemard V. et al (2010) An agonistic mAb directed to the TrkC receptor juxtamembrane region defines a trophic hot spot and interactions with p75 coreceptors. Dev Neurobiol. 2010 Feb 15;70(3):150-64.
2. Esteban PF et al (2006) A kinase-deficient TrkC receptor isoform activates Arf6-Rac1 signaling through the scaffold protein tamalin. J Cell Biol. 2006 Apr 24;173(2):291-9
3. Baker KA. et al (2007) Dorsal column sensory axons lack TrkC and are not rescued by local neurotrophin-3 infusions following spinal cord contusion in adult rats. Exp Neurol. 2007 May;205(1):82-91
4. Zaccaro M. et al. (2005) Chemistry and Biology 12; pp. 1015-1028 (2005)
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