| Catalogue No.: |
M-1161-100 |
| Batch No.: |
See product label |
| Unit size: |
100 µg |
| Antigen: |
Pig eye lens vimentin |
| Other Names: |
VIM; |
| Accession: |
P08670 VIME_HUMAN; P02543 VIME_PIG; |
| Description: |
Vimentins are class-III intermediate filaments specific to mesenchymal tissue. Vimentin is an important cytoskeletal component responsible for maintaining cell integrity and has a probable role in the intracellular transport of proteins such as lipoproteins between the nucleus and plasma membrane. Immunohistochemical staining for Vimentin is characteristic of sarcomas. |
| Produced in: |
Mouse |
| Purity: |
IgG |
| Applications: |
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/ml is recommended for WB. Human vimentin (precursor) has a predicted length of 466 residues and MW of 54 kDa. A concentration of 1.5-2.5 µg/ml is recommended to detect the protein in formalin fixed and paraffin embedded tissues. |
| Specificity: |
The specificity of this antibody has been confirmed by WB and IHC against the antigen. |
| Cross-reactivity: |
Human; mouse; rat; |
| Blast it: |
If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example. |
| Form: |
Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.2mg NaN3 |
| Reconstitution: |
Reconstitute in 1 ml of PBS (pH 7.4) to achieve an antibody concentration of 100 µg/ml. Centrifuge to remove any insoluble material. |
| Storage: |
At least 12 months after purchase at 2 - 4°C (lyophilized formulations).
After reconstitution, aliquot and store at -20°C for a higher stability.
Avoid freeze-thaw cycles |
| Expiry Date: |
12 months after purchase |
