| Catalogue No. |
PE-019-500 |
| Description |
Mouse NGF (2.5S) was isolated from mouse submaxillary glands by method of Mobley et al (1976) and is a form of beta-NGF that has identical biological properties. NGF is known to regulate the survival and development of certain sympathetic and sensory neurons. It is a dimer with 2 identical polypeptide chains and dimeric molecular weight of approximately 26,500 Da. Isolation and purification of NGF from mouse submaxillary glands yields preparations of NGF (2.5S) with identical biological activity but with cleavages at the amino terminus (with the loss of 8 amino acids) and/or at the carboxy-terminus (with the loss of arginine). These preparations are named 2.5 NGF (see reference below). |
| Batch No. |
See product label |
| Unit size |
500 µg |
| Other Names |
mouse NGF; beta NGF |
| Accession |
NGF_MOUSE |
| Produced in |
Mouse |
| Molecular Weight |
The homodimer has a molecular weight of ~26KDa and the monomer ~13KDa as confirmed by SDS PAGE. |
| Purity |
Greater than 90% (as determined by SDS electrophoresis) |
| Biol. activity |
Tested by stimulation of 8 day old chick embryo dorsal root ganglia. |
| Form |
Lyophilised from 0.1M acetic acid under dry N2 with vacuum. Appears as near-white powder. Solution sterile filtered prior to drying. |
| Reconstitution |
Reconstitute with the addition of 500 µl sterile water or phosphate buffered saline and aliquot. |
| Storage |
Short term: -20ºC. Avoid repeated freezing and thawing. |
| Expiry Date |
12 months upon purchase |
| Specific References |
1. E.M. Forbes et al (2012) Calcium and cAMP Levels Interact to Determine Attraction versus Repulsion in Axon Guidance.
Neuron. 2012 May 10;74(3):490-503.
|
| General References |
1. Mobley WC, Schenker A, Shooter EM (1976)
Biochemistry. 15(25) pp. 5543-52. |
