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Alpha-Synuclein Rapid ELISA Kit: Human (2 Plates)

$767.00USD


Catalogue No. BEK-2216-2P
  Find a 1 plate size of this product here. Enquire about bulk discounts for ten or more plates, at sales@biosensis.com
Description The Biosensis Alpha-Synuclein RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of human alpha-synuclein in less than 4 hours in human citrate-plasma, serum and CSF only if used as directed. Please refer to the kit protocol for specific use instructions for blood and CSF application.

This ELISA kit consists of a pre-coated sheep polyclonal anti-alpha-synuclein (aa: 116-131) capture antibody, a biotinylated mouse monoclonal anti-alpha-synuclein detection antibody (aa: 61-95) and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of alpha-synuclein present in samples and protein standards. A human alpha-synuclein positive control (QC sample) is provided to assure consistent assay performance.

This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Batch No. Refer to the product label.
Antigen Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. Alpha synuclein has been associated with several neurodegenerative diseases. A point mutation in the gene coding for the alpha-synuclein protein was the first discovery linking this protein to a rare familial form of Parkinson's disease (PD). Subsequently, other mutations in the alpha-synuclein gene have been identified in familial PD. The aggregated proteinaceous inclusions called Lewy bodies found in PD and cortical Lewy body dementia (LBD) were discovered to be predominantly alpha-synuclein. Aberrant aggregation of alpha-synuclein has been detected in an increasing number of neurodegenerative diseases, collectively known as synucleopathies. Alpha-synuclein exists physiologically in both soluble and membrane-bound states, in unstructured and alpha-helical conformations, respectively. The physiological function of alpha-synuclein appears to require its translocation between these subcellular compartments and interconversion between the 2 conformations. Abnormal processing of alpha-synuclein is predicted to lead to pathological changes in its binding properties and function.
Other Names Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP
Accession P37840 SYUA_HUMAN
Produced in The standard for this kit is recombinant alpha-synuclein protein produced in and purified from E.coli.
Applications ELISA. The purpose of this kit is the in-vitro quantitative determination of human alpha-synuclein in samples such as citrate-plasma and serum.
Specificity Human alpha-synuclein. While not directly tested in the ELISA, beta- and gamma-synuclein is not expected to cross-react due to the specificity of both capture and detection antibody for alpha-synuclein. Individual alpha-synuclein isoforms have not yet been tested, but it is expected that this ELISA kit detects monomeric, oligomeric and phosphorylated alpha-synuclein isoforms.
Cross-reactivity While not directly tested in the ELISA, mouse and rat alpha-synuclein are expected to cross-react due to species cross-reactivity of both capture and detection antibody.
Storage Store at 2-8C.
Expiry Date 12 months from purchase.
Kit components The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Range 0.16 - 10 ng/mL
Sensitivity Typical limit of detection (LOD) for alpha-synuclein is < 100 pg/mL, determined as alpha-synuclein concentration at blank OD plus 3x standard deviation of blank OD (n=10).
Kit protocol Click to download the kit protocolBEK-2216_as_at_December2019.pdf
MSDS Click to download the Material Safety Data SheetMSDS_Rapid_ELISA_as_at_Aug2017.pdf
Images (click to zoom)
Alpha-Synuclein Rapid ELISA Kit: Human (2 Plates) This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.
Alpha-Synuclein Rapid ELISA Kit: Human (2 Plates) Parallelism of calibration curve and diluted citrate-plasma samples from healthy individuals demonstrates accurate quantification of alpha-synuclein in human citrate-plasma.
Alpha-Synuclein Rapid ELISA Kit: Human (2 Plates) Accurate quantification of alpha-synuclein in human serum from healthy individuals across four dilutions.
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

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