biosensis fine bioscience tools - antibodies, elisa kits, proteins, toxins and peptides An Australia-US partnershipPhone: 1800 605 5127 in the US, or +61 8 8352 7711 for international enquiries to Australia.
HomeAbout BiosensisBiosensis distributorsResourcesHelp deskordering from BiosensisContact us
 


Technical Notes
 
View our tech notes
 
 
Follow us...
 
Google+ Follow us on Twitter

Find us on Facebook

Sign up for Biosensis newsletters
Visit our newsletter archive
 
 

Amylo-Glo RTD Amyloid Plaque Stain Reagent

$247.00USD


Catalogue No. TR-300-AG
Description Amylo-Glo® RTD™ “Ready to Dilute” Staining reagent is designed to stain amyloid plaques in tissue sections. This novel marker has several advantages over other conventional markers such as Thioflavin S and Congo Red because of its unique chemical and spectral properties. (L. Schmued et al. (2012) J.Neuroscience Methods 209:120– 126). Using Amylo-Glo® results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. Its brightness makes it ideal for low magnification quantification studies, while its unique excitation/emission profile and mild staining conditions makes it ideal for combination for multiple immunofluorescent labeling studies. Amylo-Glo® RTD™ is compatible with fresh, frozen, and formalin-fixed immunohistochemistry or cytochemistry, and it is particularly good for confocal and multiple labeling because of its high fluorescent intensity and high resistance to photo-bleaching. Moreover because Amylo-Glo® fluoresces in the UV channel, double and triple labeling experiments can be performed very easily (see protocol).
Related products Amylo-Glo / EB kit catalog number TR-400-AG
Batch No. See vial label
Other Names AmyloGlo
Compound Name Compound: Amylo-Glo; Classification: Styrylbenzene derivative; Appearance: Yellow solution; Molecular Weight: 392; Filter system for visualizing: UV
Purity Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of two fluorescent isomers. No amount of starting material was detected.
Applications Staining of amyloid plaques in human and animal tissues, see included protocol
Specificity amyloid plaques both intraneuronal and vascular
Appearance Excitation Peak: 334; Emission Peak: 533 nm - unbound, 438 nm when bound to amyloid. To visualize Amylo-glo in tissue, UV light is required. For example, Amylo-Glo tissue can be examined using an epifluoresent microscope with UV (Nikon UV-2A) filter cube. Excitation (325-375nm) Emission (400-450nm) is typical. Also note, it is not uncommon for Amylo-Glo to appear light yellow when examined by eye, yet appear a light blue color when photographed.
Reconstitution Ready to dilute per protocol; 100X
Storage The stock solution can be stored for up to 6 months after date of receipt at 4°C protected from light. No preservatives. Use sterile technique when handling and proper laboratory procedures.
Expiry Date 6 months from date of purchase
Specific References Palombo F (2017) "Detection of Aβ plaque-associated astrogliosis in Alzheimer’s disease brain by spectroscopic imaging and immunohistochemistry."Analyst. [Epub ahead of print] 2017; Application: IF Species: Mouse

Abud EM (2017) "Generation of Human Microglia from Induced Pluripotent Stem Cells to Study Innate Immunity in Neurological Diseases."PhD Thesis. 2017; Application: IF Species: Mouse

Abud EM et al. (2017) "iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases."Neuron. 2017; 49(2):278-93 Application: IF Species: Mouse

Solomon IH et al. (2017) "Brain and liver pathology, amyloid deposition, and interferon responses among older HIV-positive patients in the late HAART era."BMC Infect Dis. 2017; 17(1):151 Application: IF Species: Human

Xu F et al. (2016) "Cerebral vascular amyloid seeds drive amyloid β-protein fibril assembly with a distinct anti-parallel structure."Nat Commun. 2016; 7:13527. Application: IF Species: Mouse

Katsouri L et al. (2016) "PPARγ-coactivator-1α gene transfer reduces neuronal loss and amyloid-β generation by reducing β-secretase in an Alzheimer’s disease model ."Proc Natl Acad Sci USA. 2016; 113(43):12292-97. Application: IF Species: Mouse

Esposito G et al. (2016) "Autologous transplantation of intestine-isolated glia cells improves neuropathology and restores cognitive deficits in β amyloid-induced neurodegeneration."Sci Rep. 2016; 6: 22605. Application: IF Species: Rat

Marsh SE et al. (2016) "The adaptive immune system restrains Alzheimer's disease pathogenesis by modulating microglial function." Proc Natl Sci USA. Feb 16. pii: 201525466. Application: IF Species: Hu Fibrillar amyloid visualization.

Kim YH et al. (2015) "A 3D human neural cell culture system for modeling Alzheimer's disease." Nat Protoc. Jul;10(7):985-1006. Application: IF Species: Hu, Human neural stem-cell-derived three-dimensional (3D) culture system.

Nijholt DA et al. (2015) "Pregnancy Zone Protein is Increased in the Alzheimer's Disease Brain and Associates with Senile Plaques." J Alzheimer's Disease. 46(1):227-38. Application: IF Species: Hu

Kamphuis W et al. (2015) "GFAP and vimentin deficiency alters gene expression in astrocytes and microglia in wild-type mice and changes the transcriptional response of reactive glia in mouse model for Alzheimer's disease." Glia. Jun;63(6):1036-56. Application: IF Species: Mouse

Choi SH et al. (2014) "A three-dimensional human neural cell culture model of Alzheimer's disease." Nature Oct 12. doi: 10.1038/nature1380. Application: IF Species: Hu, Human neural stem-cell-derived three-dimensional (3D) culture system.

Niedowicz DM et al. (2014). "Obesity and diabetes cause cognitive dysfunction in the absence of accelerated beta-amyloid deposition in a novel murine model of mixed or vascular dementia." Acta Neuropathol Commun. 2014 Jun 10;2:64.
General References L. Schmued et al. (2012) J.Neuroscience Methods 209:120– 126
Kit Components 5 mL of 100X Amylo-Glo RTD™ (A-G RTD™) solution
Reagent Kit protocol Click to download the protocolTR-300-AG_as_at_Apr2014_protocol.pdf
MSDS Click to download the Material Safety Data SheetMSDS_TR-300-AG_as_at_July2015.pdf
Images (click to zoom)
Amylo-Glo RTD Amyloid Plaque Stain Reagent This triple exposure allows for the simultaneous localization of Amylo-Glo® positive amyloid plaques (blue), GFAP positive hypertrophied astrocytes (green) and activated microglia (red) in the hippocampus of the AD/Tg mouse. Combined UV, blue and green light illumination.
Amylo-Glo RTD Amyloid Plaque Stain Reagent Amylo-Glo UV illumination only
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

Customers who bought this product also purchased
Fluoro-Jade C  (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating Neurons
Fluoro-Jade C (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating Neurons
MOAB-2 Mouse Monoclonal antibody to Amyloid beta peptide (A beta 40/42), purified
MOAB-2 Mouse Monoclonal antibody to Amyloid beta peptide (A beta 40/42), purified
Rabbit polyclonal antibody to Sodium Channel subunit beta-1 (73-95): Affinity purified
Rabbit polyclonal antibody to Sodium Channel subunit beta-1 (73-95): Affinity purified

Chicken polyclonal antibody to Adiponectin (230-244): Affinity purified
Chicken polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP)
Chicken polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP)

Offices in the USA and Australia
My Ice Bucket  
 
0 items
 
 
Currencies
 
  
 
 
Email This Page
 
 
Tell someone you know about this product.
 
 
We Accept PayPal
 
You can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_ID
Click to make a payment


Official PayPal Seal