||Amylo-Glo® RTD™ “Ready to Dilute” Staining reagent is designed to stain amyloid plaques in tissue sections. This novel marker has several advantages over other conventional markers such as Thioflavin S and Congo Red because of its unique chemical and spectral properties. (L. Schmued et al. (2012) J.Neuroscience Methods 209:120– 126). Using Amylo-Glo® results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. Its brightness makes it ideal for low magnification quantification studies, while its unique excitation/emission profile and mild staining conditions makes it ideal for combination for multiple immunofluorescent labeling studies. Amylo-Glo® RTD™ is compatible with fresh, frozen, and formalin-fixed immunohistochemistry or cytochemistry, and it is particularly good for confocal and multiple labeling because of its high fluorescent intensity and high resistance to photo-bleaching. Moreover because Amylo-Glo® fluoresces in the UV channel, double and triple labeling experiments can be performed very easily (see protocol).
||Amylo-Glo / EB kit catalog number TR-400-AG
||See vial label
||Compound: Amylo-Glo; Classification: Styrylbenzene derivative; Appearance: Yellow solution; Molecular Weight: 392; Filter system for visualizing: UV
||Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of two fluorescent isomers. No amount of starting material was detected.
||Staining of amyloid plaques in human and animal tissues, see included protocol
||amyloid plaques both intraneuronal and vascular
||Excitation Peak: 334; Emission Peak: 533 nm - unbound, 438 nm when bound to amyloid.
To visualize Amylo-glo in tissue, UV light is required. For example,
Amylo-Glo tissue can be examined using an epifluoresent microscope with UV (Nikon UV-2A) filter cube. Excitation (325-375nm) Emission (400-450nm) is typical. Also note, it is not uncommon for Amylo-Glo to appear light yellow when examined by eye, yet appear a light blue color when photographed.
||Ready to dilute per protocol; 100X
||The stock solution can be stored for up to 6 months after date of receipt at 4°C protected from light. No preservatives. Use sterile technique when handling and proper laboratory procedures.
||6 months from date of purchase
||Palombo F (2017) "Detection of Aβ plaque-associated astrogliosis in Alzheimer’s disease brain by spectroscopic imaging and immunohistochemistry."Analyst. [Epub ahead of print] 2017; Application: IF Species: Mouse
Abud EM (2017) "Generation of Human Microglia from Induced Pluripotent Stem Cells to Study Innate Immunity in Neurological Diseases."PhD Thesis. 2017; Application: IF Species: Mouse
Abud EM et al. (2017) "iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases."Neuron. 2017; 49(2):278-93 Application: IF Species: Mouse
Solomon IH et al. (2017) "Brain and liver pathology, amyloid deposition, and interferon responses among older HIV-positive patients in the late HAART era."BMC Infect Dis. 2017; 17(1):151 Application: IF Species: Human
Xu F et al. (2016) "Cerebral vascular amyloid seeds drive amyloid β-protein fibril assembly with a distinct anti-parallel structure."Nat Commun. 2016; 7:13527. Application: IF Species: Mouse
Katsouri L et al. (2016) "PPARγ-coactivator-1α gene transfer reduces neuronal loss and amyloid-β generation by reducing β-secretase in an Alzheimer’s disease model ."Proc Natl Acad Sci USA. 2016; 113(43):12292-97. Application: IF Species: Mouse
Esposito G et al. (2016) "Autologous transplantation of intestine-isolated glia cells improves neuropathology and restores cognitive deficits in β amyloid-induced neurodegeneration."Sci Rep. 2016; 6: 22605. Application: IF Species: Rat
Marsh SE et al. (2016) "The adaptive immune system restrains Alzheimer's disease pathogenesis by modulating microglial function."
Proc Natl Sci USA. Feb 16. pii: 201525466. Application: IF Species: Hu Fibrillar amyloid visualization.
Kim YH et al. (2015) "A 3D human neural cell culture system for modeling Alzheimer's disease."
Nat Protoc. Jul;10(7):985-1006. Application: IF Species: Hu, Human neural stem-cell-derived three-dimensional (3D) culture system.
Nijholt DA et al. (2015) "Pregnancy Zone Protein is Increased in the Alzheimer's Disease Brain and Associates with Senile Plaques."
J Alzheimer's Disease. 46(1):227-38. Application: IF Species: Hu
Kamphuis W et al. (2015) "GFAP and vimentin deficiency alters gene expression in astrocytes and microglia in wild-type mice and changes the transcriptional response of reactive glia in mouse model for Alzheimer's disease."
Glia. Jun;63(6):1036-56. Application: IF Species: Mouse
Choi SH et al. (2014) "A three-dimensional human neural cell culture model of Alzheimer's disease."
Nature Oct 12. doi: 10.1038/nature1380. Application: IF Species: Hu, Human neural stem-cell-derived three-dimensional (3D) culture system.
Niedowicz DM et al. (2014). "Obesity and diabetes cause cognitive dysfunction in the absence of accelerated beta-amyloid deposition in a novel murine model of mixed or vascular dementia." Acta Neuropathol Commun. 2014 Jun 10;2:64.
||L. Schmued et al. (2012) J.Neuroscience Methods 209:120– 126
||5 mL of 100X Amylo-Glo RTD™ (A-G RTD™) solution
|Reagent Kit protocol